Husma Amrah
Department of Aquaculture, Faculty of Fishery and Marine Science, Indonesia Muslim University, Makassar-90231, South Sulawesi, Indonesia.
Pak J Biol Sci. 2017;20(7):336-342. doi: 10.3923/pjbs.2017.336.342.
Marosatherina ladigesi, including native fish in South Sulawesi Indonesia, are vulnerable and must be preserved. The objective of the study was to examine known food consumption, survival, growth and the activities of digestive enzymes of M. ladigesi larvae.
The larvae were obtained from natural spawning and the mixed live feed: Infusoria sp., Daphnia sp. and Artemia salina were given twice a day. Feed intake, growth, survival, activities of protease, lipase and amylase enzymes were observed in ages 3, 5, 10, 15, 20, 25, 30, 35 Days after Hatching (DAH). Data were analyzed by following a complete randomized trial design.
Activities of protease, amylase and lipase enzymes have been detected since 3 DAH. The protease enzyme activity was obtained at 0.438 U mL min-1 at 35 DAH, while amylase and lipase activities fluctuated. Activities of amylase and lipase reached 0105 and 0.093 U mL-1 min-1 at 15 DAH, respectively. The activity of the enzyme increased at the same age of 10-15 DAH larvae entering a phase of flexion.
It was concluded that the use of mixed live feed Infusoria sp., Daphnia sp. and A. salina contribute to enzyme protease, lipase and amylase activities during the ontogeny stages of M. ladigesi. The highest activity of protease suggests that the fish have carnivorous feeding habits. The results of this study can be used to improve the maintenance technique of M. ladigesi larvae in order to accelerate growth and improve survival as an effort to increase the production of M. ladigesi.
拉氏唇银汉鱼是印度尼西亚南苏拉威西岛的本土鱼类，属于易危物种，必须加以保护。本研究的目的是考察拉氏唇银汉鱼幼鱼已知的食物消耗、存活、生长情况以及消化酶的活性。
幼鱼取自自然产卵，每天投喂两次混合活饵：纤毛虫、水蚤和卤虫。在孵化后3、5、10、15、20、25、30、35天（DAH）观察摄食量、生长、存活情况以及蛋白酶、脂肪酶和淀粉酶的活性。数据采用完全随机试验设计进行分析。
自孵化后3天起就检测到了蛋白酶、淀粉酶和脂肪酶的活性。在孵化后35天，蛋白酶活性为0.438 U mL min-1，而淀粉酶和脂肪酶的活性有所波动。在孵化后15天，淀粉酶和脂肪酶的活性分别达到0.105和0.093 U mL-1 min-1。在幼鱼进入弯曲期的10 - 15天龄时，酶活性增加。
得出的结论是，使用纤毛虫、水蚤和卤虫的混合活饵有助于拉氏唇银汉鱼个体发育阶段的蛋白酶、脂肪酶和淀粉酶活性。蛋白酶的最高活性表明该鱼具有肉食性摄食习性。本研究结果可用于改进拉氏唇银汉鱼幼鱼的养殖技术，以加速生长并提高存活率，从而增加拉氏唇银汉鱼的产量。
