WPI-Advanced Institute for Materials Research, Tohoku University , Sendai 980-8577, Japan.
Department of Medicine, Center for Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School , Cambridge, Massachusetts 02139, United States.
ACS Appl Mater Interfaces. 2017 Dec 13;9(49):42444-42458. doi: 10.1021/acsami.7b03979. Epub 2017 Dec 4.
In this study, composite gelatin-polyaniline (PANI) nanofibers doped with camphorsulfonic acid (CSA) were fabricated by electrospinning and used as substrates to culture C2C12 myoblast cells. We observed enhanced myotube formation on composite gelatin-PANI nanofibers compared to gelatin nanofibers, concomitantly with enhanced myotube maturation. Thus, in myotubes, intracellular organization, colocalization of the dihydropyridine receptor (DHPR) and ryanodine receptor (RyR), expression of genes correlated to the excitation-contraction (E-C) coupling apparatus, calcium transients, and myotube contractibility were increased. Such composite material scaffolds combining topographical and electrically conductive cues may be useful to direct skeletal muscle cell organization and to improve cellular maturation, functionality, and tissue formation.
在这项研究中，通过静电纺丝制备了掺杂樟脑磺酸（CSA）的复合明胶-聚苯胺（PANI）纳米纤维，并将其用作培养 C2C12 成肌细胞的基质。我们观察到，与明胶纳米纤维相比，复合明胶-PANI 纳米纤维上的肌管形成得到了增强，同时肌管成熟也得到了增强。因此，在肌管中，细胞内组织、二氢吡啶受体（DHPR）和兰尼碱受体（RyR）的共定位、与兴奋-收缩（E-C）耦联装置相关的基因表达、钙瞬变和肌管收缩性均增加。这种结合了形貌和导电性线索的复合材料支架可能有助于指导骨骼肌细胞的组织，并改善细胞成熟度、功能和组织形成。
