Pedroni P, Riboli B, de Ferra F, Grandi G, Toma S, Aricò B, Rappuoli R
Enricherche S. p.A., Department of Molecular Biology, Milan, Italy.
Mol Microbiol. 1988 Jul;2(4):539-43. doi: 10.1111/j.1365-2958.1988.tb00061.x.
A search for pilin genes in a Bordetella pertussis (Bp) genomic library has led to the identification of several clones which hybridize to synthetic oligonucleotides with sequences derived from amino acid sequences of Bp fimbrial subunits. One of these clones (corresponding to a gene we have named fimX) contains an open reading frame encoding a protein with a molecular weight of about 20 kD and a sequence similar but not identical to the fimbrial subunit fim2 and to other fimbrial protein sequences. In this communication we present the cloning and nucleotide sequence of the fimX gene and its homology to the fim2 gene. A genomic analysis on the positional relationship between the two genes is also presented.
在百日咳博德特氏菌（Bp）基因组文库中搜索菌毛蛋白基因，已鉴定出几个与合成寡核苷酸杂交的克隆，这些寡核苷酸的序列源自Bp菌毛亚基的氨基酸序列。其中一个克隆（对应于我们命名为fimX的基因）包含一个开放阅读框，编码一种分子量约为20 kD的蛋白质，其序列与菌毛亚基fim2以及其他菌毛蛋白序列相似但不相同。在本通讯中，我们展示了fimX基因的克隆、核苷酸序列及其与fim2基因的同源性。还对这两个基因之间的位置关系进行了基因组分析。
