Livey I, Duggleby C J, Robinson A
Molecular Genetics Laboratory, PHLS, Centre for Applied Microbiology and Research, Salisbury, Wiltshire, UK.
Mol Microbiol. 1987 Sep;1(2):203-9. doi: 10.1111/j.1365-2958.1987.tb00513.x.
An oligonucleotide probe complementary to the beginning of the gene encoding the serotype 2(ST2) fimbrial subunit of Bordetella pertussis was synthesized and a cloned DNA fragment hybridizing with the probe identified and sequenced. Several lines of evidence indicate that an open reading frame with coding information for a polypeptide of 207 amino acids, including a 26-amino-acid signal sequence, is the ST2 gene. The protein deduced from the nucleotide sequence shows good agreement with the NH2-terminal amino acid sequence, amino acid composition and molecular weight of the purified fimbrial subunit. In addition, the proposed ST2 subunit is shown to have homology with other fimbrial subunits.
合成了一种与百日咳博德特氏菌2型（ST2）菌毛亚基编码基因起始部分互补的寡核苷酸探针，并鉴定和测序了与该探针杂交的克隆DNA片段。多条证据表明，一个开放阅读框是ST2基因，它编码一个含207个氨基酸的多肽，包括一个26个氨基酸的信号序列。从核苷酸序列推导的蛋白质与纯化菌毛亚基的氨基末端氨基酸序列、氨基酸组成和分子量高度一致。此外，推测的ST2亚基与其他菌毛亚基具有同源性。
