School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea.
Graduate School of Analytical Science and Technology, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea.
Mater Sci Eng C Mater Biol Appl. 2018 Jan 1;82:19-24. doi: 10.1016/j.msec.2017.08.057. Epub 2017 Aug 12.
The application of pulsed and continuous wave (CW) lasers in nanomedicine has increased significantly over the last decade. Near infrared (NIR) lasers can be used for the precise control of drug release at the target site in a non-invasive manner. In this study, we have prepared nanographene oxide (nGO, size 40nm) integrated liposomes (size ~900nm). The nGOs were not simply adsorbed onto the liposome surface but was embedded inside the liposomes as characterized by cryo-TEM, selected area electron diffraction (SAED), and fluorescence quenching studies. The embedded nGOs could act as a molecular switch for NIR light controlled drug release from the liposomes. Calcein was encapsulated into the liposome as a model drug to evaluate the efficiency of light controlled release. An on-demand pulsatile drug release was achieved by irradiation of CW/pulsed NIR lasers into the nGO-liposome suspension. Triggering with a pulsed laser resulted in larger release of calcein with a minimal temperature increase (2°C) of the liposome solution, compared to lower release rate and a significant temperature increase (~8°C) by a CW laser with the same light energy, suggesting two separate mechanisms and different potential applications depending on the laser type.
在过去的十年中，脉冲和连续波（CW）激光在纳米医学中的应用显著增加。近红外（NIR）激光可以用于非侵入性地精确控制靶部位的药物释放。在这项研究中，我们制备了纳米氧化石墨烯（nGO，尺寸约为 40nm）与脂质体（尺寸约为 900nm）的复合物。nGO 并未简单地吸附在脂质体表面，而是嵌入在脂质体内部，这可通过低温透射电子显微镜、选区电子衍射（SAED）和荧光猝灭研究来表征。嵌入的 nGO 可以作为分子开关，用于 NIR 光控制脂质体中的药物释放。将 calcein 包封在脂质体中作为模型药物，以评估光控释放的效率。通过将 CW/脉冲 NIR 激光照射到 nGO-脂质体悬浮液中，实现了按需脉冲式药物释放。与 CW 激光相比，使用脉冲激光触发可导致 calcein 的释放量更大，脂质体溶液的温度升高（约 2°C）最小，而 CW 激光的释放速率较低，温度升高（约 8°C）显著，这表明存在两种不同的机制和不同的潜在应用，具体取决于激光类型。
