Blumhorst Michael R, Mahan Travis, Stanley Kathryn, Griffith Aaron, Collison Mark W
Research Division, Archer Daniels Midland Company, James R. Randall Research Center, 1001 N. Brush College Road, Decatur, IL 62521 USA.
J Am Oil Chem Soc. 2017;94(10):1323-1328. doi: 10.1007/s11746-017-3039-2. Epub 2017 Aug 30.
AOCS Official Method Ce 6-86 "Antioxidants, Liquid Chromatographic Method" was originally developed to confirm the correct antioxidant was added at the specified concentration to refined oils. Today, this method is increasingly utilized to validate that antioxidants are absent from oil products. False positive results can have a significant impact on the ability to sell products in specific markets and can impart additional business expenditures for conclusive secondary analyses. In the current work, quantification of -butylhydroquinone (TBHQ) in crude canola/rapeseed oil using liquid chromatography (LC) with ultraviolet (UV) detection was compromised by an interfering peak. Analyses using liquid chromatography-mass spectrometry (GC-MS) and high-resolution accurate mass LC-MS identified the interferent as 2,6-dimethoxy-4-vinylphenol (canolol), an endogenous compound present in crude canola/rapeseed oil. Resolution of canolol and TBHQ using LC-UV can be achieved via minor modification of the chromatographic conditions.
美国油脂化学家协会（AOCS）官方方法Ce 6-86《抗氧化剂，液相色谱法》最初是为了确认在精炼油中是否按规定浓度添加了正确的抗氧化剂而制定的。如今，该方法越来越多地用于验证油品中是否不存在抗氧化剂。假阳性结果可能会对产品在特定市场的销售能力产生重大影响，并可能导致进行确定性二次分析的额外商业支出。在当前的工作中，使用带紫外（UV）检测的液相色谱（LC）对粗制油菜籽/菜籽油中的叔丁基对苯二酚（TBHQ）进行定量时，受到了一个干扰峰的影响。使用气相色谱-质谱联用（GC-MS）和高分辨率精确质量液相色谱-质谱联用（LC-MS）分析确定该干扰物为2,6-二甲氧基-4-乙烯基苯酚（油菜素），这是粗制油菜籽/菜籽油中存在的一种内源性化合物。通过对色谱条件进行微小修改，可以使用LC-UV分离油菜素和TBHQ。
