El-Mowafy Somaia A, Abd El Galil Khaled H, Habib El-Sayed E, Shaaban Mona I
Microbiology Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516 Egypt.
Pharmaceutical Sciences Department, Faculty of Pharmacy, Beirut Arab University, Beirut, Lebanon. Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura, 35516 Egypt.
Afr Health Sci. 2017 Mar;17(1):199-207. doi: 10.4314/ahs.v17i1.25.
The virulence factors of are under the control of quorum sensing (QS) signals. Hence, interference with QS prevents its pathogenesis.
The aim of the present research is to assess the influence of some β-lactam antibiotics on cell communication and the release of different virulence factors.
The minimal inhibitory concentrations of ceftazidime, cefepime and imipenem were evaluated by microbroth dilution method. The effect of sub-inhibitory concentration of the tested antibiotics on QS signals was investigated using reporter strain assay. In addition, different virulence factors (elastase, protease, pyocyanin and hemolysin) were estimated in the presence of their sub-inhibitory concentrations.
Low concentrations of ceftazidime, cefepime and imipenem caused significant elimination of the QS signals 3OH-C12-HSL and C4-HSL up to 1/20 MIC. Furthermore, low concentrations of the tested antimicrobials suppressed virulence factors elastase and hemolysin. Moreover, 1/20 of their MICs reduced elastase, protease, pyocyanin and hemolysin.
Utilization of β-lactam antibiotics at low concentrations could be an effective approach for prevention and treatment of infection.
[病原体名称]的毒力因子受群体感应（QS）信号控制。因此，干扰群体感应可预防其发病机制。
本研究旨在评估某些β-内酰胺类抗生素对细胞通讯及不同毒力因子释放的影响。
采用微量肉汤稀释法评估头孢他啶、头孢吡肟和亚胺培南的最低抑菌浓度。使用报告菌株分析法研究受试抗生素亚抑菌浓度对群体感应信号的影响。此外，在其亚抑菌浓度存在的情况下评估不同毒力因子（弹性蛋白酶、蛋白酶、绿脓菌素和溶血素）。
低浓度的头孢他啶、头孢吡肟和亚胺培南可显著消除群体感应信号3OH-C12-HSL和C4-HSL，直至1/20 MIC。此外，低浓度的受试抗菌药物抑制毒力因子弹性蛋白酶和溶血素。而且，其1/20 MIC可降低弹性蛋白酶、蛋白酶、绿脓菌素和溶血素。
低浓度使用β-内酰胺类抗生素可能是预防和治疗[病原体名称]感染的有效方法。
