Amaike Kazuma, Tamura Tomonori, Hamachi Itaru
Graduate School of Engineering, Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Chem Commun (Camb). 2017 Nov 14;53(88):11972-11983. doi: 10.1039/c7cc07177a. Epub 2017 Oct 13.
Endogenous protein labeling is one of the most invaluable methods for studying the bona fide functions of proteins in live cells. However, multi-molecular crowding conditions, such as those that occur in live cells, hamper the highly selective chemical labeling of a protein of interest (POI). We herein describe how the efficient coupling of molecular recognition with a chemical reaction is crucial for selective protein labeling. Recognition-driven protein labeling is carried out by a synthetic labeling reagent containing a protein (recognition) ligand, a reporter tag, and a reactive moiety. The molecular recognition of a POI can be used to greatly enhance the reaction kinetics and protein selectivity, even under live cell conditions. In this review, we also briefly discuss how such selective chemical labeling of an endogenous protein can have a variety of applications at the interface of chemistry and biology.
内源性蛋白质标记是研究活细胞中蛋白质真实功能最有价值的方法之一。然而，多分子拥挤条件，如活细胞中出现的条件，会妨碍对目标蛋白质（POI）进行高度选择性的化学标记。我们在此描述分子识别与化学反应的有效偶联对于选择性蛋白质标记至关重要。识别驱动的蛋白质标记由一种合成标记试剂进行，该试剂包含蛋白质（识别）配体、报告标签和反应基团。即使在活细胞条件下，对POI的分子识别也可用于极大地提高反应动力学和蛋白质选择性。在本综述中，我们还简要讨论了这种内源性蛋白质的选择性化学标记如何能在化学与生物学的交叉领域有多种应用。
