Asp Patrik
Albert Einstein College of Medicine, Michael F. Price Center, 1300 Morris Park Avenue, Bronx, NY, 10461, USA.
Methods Mol Biol. 2018;1689:29-42. doi: 10.1007/978-1-4939-7380-4_3.
Chromatin immunoprecipitation (ChIP) coupled with quantitative PCR (qPCR) has in the last 15 years become a basic mainstream tool in genomic research. Numerous commercially available ChIP kits, qPCR kits, and real-time PCR systems allow for quick and easy analysis of virtually anything chromatin-related as long as there is an available antibody. However, the highly accurate quantitative dimension added by using qPCR to analyze ChIP samples significantly raises the bar in terms of experimental accuracy, appropriate controls, data analysis, and data presentation. This chapter will address these potential pitfalls by providing protocols and procedures that address the difficulties inherent in ChIP-qPCR assays.
在过去15年里，染色质免疫沉淀（ChIP）与定量PCR（qPCR）相结合已成为基因组研究中的一种基本主流工具。众多市售的ChIP试剂盒、qPCR试剂盒和实时PCR系统，只要有可用抗体，就能快速简便地分析几乎任何与染色质相关的内容。然而，使用qPCR分析ChIP样品所增加的高度精确的定量维度，在实验准确性、适当的对照、数据分析和数据呈现方面显著提高了标准。本章将通过提供解决ChIP-qPCR分析中固有困难的方案和程序来应对这些潜在的陷阱。
