通过微球菌核酸酶消化实现的DNA可及性

DNA Accessibility by MNase Digestions.

作者信息

Farrants Ann-Kristin Östlund

机构信息

Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Svante Arrhenius väg 20C, 106 91, Stockholm, Sweden.

出版信息

Methods Mol Biol. 2018;1689:77-82. doi: 10.1007/978-1-4939-7380-4_7.

DOI:10.1007/978-1-4939-7380-4_7

PMID:29027166

Abstract

Micrococcal nuclease (MNase) digestion of chromatin cuts linker DNA between neighboring nucleosomes and in this way generates mononucleosomes. The protected fragments can then be analyzed by genome-wide sequencing techniques or by quantitative PCR to obtain information about the positions of nucleosomes in the chromatin. Nucleosomes are differentially sensitive to MNase digestion, which means that titrations of MNase should be performed to obtain a comprehensive map of the nucleosome positions of a chromatin region or genome.

摘要

微球菌核酸酶（MNase）对染色质的消化作用会切断相邻核小体之间的连接DNA，从而产生单核小体。然后可以通过全基因组测序技术或定量PCR对受保护的片段进行分析，以获取有关染色质中核小体位置的信息。核小体对MNase消化的敏感性存在差异，这意味着应该进行MNase滴定，以获得染色质区域或基因组核小体位置的全面图谱。