Zhang Jingyi, Liu Donghao, Shi Yi, Sun Chen, Niu Muchuan, Wang Ruya, Hu Fan, Xiao Deli, He Hua
Department of Analytical Chemistry, China Pharmaceutical University, 24 Tongjia Lane, Nanjing 210009, China.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Nov 15;1068-1069:24-32. doi: 10.1016/j.jchromb.2017.09.046. Epub 2017 Oct 2.
In this research, a novel insoluble sorbent based on cyclodextrin and rigid aromatic groups tetrafluoroacetonitrile was designed for dispersive insoluble solid-phase extraction (DSPE). Due to its high adsorption capacity, this obtained polymer was applied to separation and concentration of trace quinolones in wastewater before HPLC determination. Various parameters influencing the extraction performance were studied and optimized. A DSPE approach coupled with high performance liquid chromatography was developed for the determination of four quinolones in wastewater samples. The limit of quantitation of fleroxacin, ciprofloxacin, gatifloxacin, norfloxacin were 2.67, 3.17, 4.75, 5.50ngmL, respectively. The recoveries of four quinolones range from 96.43 to 103.3% with relative standard deviations less than 4.5%. These results demonstrated that the proposed approach based on CDP was efficient, low-cost for extraction of quinolones from wastewater.
在本研究中，设计了一种基于环糊精和刚性芳香基团四氟乙腈的新型不溶性吸附剂，用于分散不溶性固相萃取（DSPE）。由于其高吸附容量，将所得聚合物应用于废水样品中痕量喹诺酮类药物的分离和富集，然后进行高效液相色谱测定。研究并优化了影响萃取性能的各种参数。建立了一种结合高效液相色谱的分散不溶性固相萃取方法，用于测定废水样品中的四种喹诺酮类药物。氟罗沙星、环丙沙星、加替沙星、诺氟沙星的定量限分别为2.67、3.17、4.75、5.50 ng/mL。四种喹诺酮类药物的回收率在96.43%至103.3%之间，相对标准偏差小于4.5%。这些结果表明，所提出的基于环糊精聚合物的方法对于从废水中萃取喹诺酮类药物是高效且低成本的。
