Danek Magdalena, Korytkowska-Wałach Anna, Barchańska Hanna
Department of Inorganic Chemistry, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, B. Krzywoustego 6 St., 44-100 Gliwice, Poland.
Department of Organic Chemistry, Bioorganic Chemistry and Biotechnology, Faculty of Chemistry, Silesian University of Technology, B. Krzywoustego 4 St., 44-100 Gliwice, Poland.
Molecules. 2021 Sep 30;26(19):5945. doi: 10.3390/molecules26195945.
Nitisinone (NTBC) is used in the treatment of disorders affecting the tyrosine pathway, including hereditary tyrosinemia type I, alkaptonuria, and neuroblastoma. An inappropriate dosage of this therapeutic drug causes side effects; therefore, it is necessary to develop a rapid and sensitive method to monitor the content of NTBC in patients' blood. This study aimed to develop anew polymeric sorbent containing β-cyclodextrin (β-CD) derivatives grafted on silica gel to effectively extract NTBC from model physiological fluids. The inclusion complex formed between β-CD and NTBC was examined by proton nuclear magnetic resonance spectroscopy. The novel sorbents with derivatives of β-CD were prepared on modified silica gel using styrene as a comonomer, ethylene glycol dimethacrylate as a crosslinking agent, and 2,2'-azo-bis-isobutyronitrile as a polymerization initiator. The obtained products were characterized via Fourier transform infrared spectroscopy and then used as sorbents as part of a solid phase extraction technique. High NTBC recovery (70%indicated that the developed polymeric sorbent may be suitable for extracting this compound from patients' blood samples.
尼替西农（NTBC）用于治疗影响酪氨酸途径的疾病，包括I型遗传性酪氨酸血症、尿黑酸尿症和成神经细胞瘤。这种治疗药物的不适当剂量会引起副作用；因此，有必要开发一种快速灵敏的方法来监测患者血液中NTBC的含量。本研究旨在开发一种新的聚合物吸附剂，该吸附剂含有接枝在硅胶上的β-环糊精（β-CD）衍生物，以有效地从模拟生理流体中提取NTBC。通过质子核磁共振光谱研究了β-CD与NTBC之间形成的包合物。以苯乙烯为共聚单体、乙二醇二甲基丙烯酸酯为交联剂、2,2'-偶氮二异丁腈为聚合引发剂，在改性硅胶上制备了具有β-CD衍生物的新型吸附剂。通过傅里叶变换红外光谱对所得产物进行了表征，然后将其用作固相萃取技术的一部分作为吸附剂。NTBC的高回收率（70%表明所开发的聚合物吸附剂可能适用于从患者血液样本中提取该化合物。
