脑胆囊收缩素和蛙皮素受体在非洲爪蟾卵母细胞中的功能表达。

Functional expression of brain cholecystokinin and bombesin receptors in Xenopus oocytes.

作者信息

Moriarty T M, Gillo B, Sealfon S, Roberts J L, Blitzer R D, Landau E M

机构信息

Department of Psychiatry, Mount Sinai School of Medicine, New York, NY 10029.

出版信息

Brain Res. 1988 Aug;464(1):75-9. doi: 10.1016/0169-328x(88)90021-6.

DOI:10.1016/0169-328x(88)90021-6

PMID:2902910

Abstract

Total RNA was extracted from 15-day-old whole rat brains. Microinjection of the RNA into Xenopus laevis oocytes induced electrophysiological responsiveness to cholecystokinin-8 (CCK) and bombesin (BBS) but not to corticotropin-releasing factor (CRF) or somatostatin. The responses to CCK and BBS were similar in shape, time course, and reversal potential to that induced by receptor mediated phospholipid breakdown and that which is induced by intracellular injection of IP3. These responses were not blocked by atropine or by mianserin, did not require extracellular Ca2+ and were completely suppressed by intracellular injection of EGTA.

摘要

从15日龄的大鼠全脑中提取总RNA。将该RNA显微注射到非洲爪蟾卵母细胞中，可诱导其对胆囊收缩素-8（CCK）和蛙皮素（BBS）产生电生理反应，但对促肾上腺皮质激素释放因子（CRF）或生长抑素无反应。对CCK和BBS的反应在形状、时间进程和反转电位方面，与受体介导的磷脂分解所诱导的反应以及细胞内注射肌醇三磷酸（IP3）所诱导的反应相似。这些反应不受阿托品或米安色林的阻断，不需要细胞外钙离子，并且通过细胞内注射乙二醇双四乙酸（EGTA）可被完全抑制。