Guy J, Herman J P, Le Moal M, Thibault J, Calas A
Laboratoire de Physiologie des Interactions Cellulaires, UA 339 CNRS, Université de Bordeaux I., Paris.
C R Seances Soc Biol Fil. 1988;182(1):67-73.
Mesencephalic fragments from 14 day old embryonic rat brain were transplanted into the third ventricle of adult rats neonatally treated with monosodium glutamate. From two to twelve months after grafting, the implanted tissue was still present in the ventricle and contained TH immunoreactive neurons which displayed a normal appearance at ultrastructural level. While endogenous TH containing neurons were still present in dopaminergic regions of the recipient hypothalamus, grafted mesencephalic fragments could survive and develop. They contained TH immunopositive most probably dopaminergic neurons which are able, in some cases, to innervate the host brain. This model should be of interest in the study of neuroendocrine functions of dopaminergic neurons.
将14日龄胚胎大鼠脑的中脑片段移植到新生期用谷氨酸单钠处理的成年大鼠的第三脑室。移植后两到十二个月，植入的组织仍存在于脑室中，并含有酪氨酸羟化酶（TH）免疫反应性神经元，这些神经元在超微结构水平上外观正常。虽然受体下丘脑的多巴胺能区域中仍存在内源性含TH神经元，但移植的中脑片段能够存活并发育。它们含有TH免疫阳性的、很可能是多巴胺能的神经元，在某些情况下，这些神经元能够支配宿主脑。该模型在多巴胺能神经元的神经内分泌功能研究中应具有重要意义。
