Fetisov S O
Ontogenez. 1994 May-Jun;25(3):21-5.
Specific antibodies were used for studying the expression of tyrosine hydroxylase (TH) and DOPA decarboxylase (DDC) in neurons of rat embryonic mediobasal hypothalamus (MBH), whose fragments were implanted into the third brain ventricle of the adult female Wistar rats. Two months after surgery, most immunopositive neurons expressed TH, smaller number of neurons expressed both TH and DDC, and few cells contained only DDC. As during development in situ, immunopositive neurons formed nuclei-like clusters located in the ventral region of the graft. This data suggest that MBH grafts contain a population of dopaminergic neurons and a population of TH-containing neurons that probably are not dopaminergic.
使用特异性抗体研究大鼠胚胎中脑基底下丘脑（MBH）神经元中酪氨酸羟化酶（TH）和多巴脱羧酶（DDC）的表达，将MBH片段植入成年雌性Wistar大鼠的第三脑室。术后两个月，大多数免疫阳性神经元表达TH，较少数量的神经元同时表达TH和DDC，仅有少数细胞仅含有DDC。如同在原位发育过程中一样，免疫阳性神经元形成位于移植物腹侧区域的核样簇。这些数据表明，MBH移植物包含一群多巴胺能神经元和一群可能不是多巴胺能的含TH神经元。
