Kieran Troy J, Gottdenker Nicole L, Varian Christina P, Saldaña Azael, Means Nicolas, Owens Darlisha, Calzada Jose E, Glenn Travis C
Department of Environmental Health Science, College of Public Health, University of Georgia, Athens, GA.
Department of Veterinary Pathology, College of Veterinary Medicine, The University of Georgia, Athens, GA.
J Med Entomol. 2017 Nov 7;54(6):1786-1789. doi: 10.1093/jme/tjx170.
Accurate blood meal identification is critical to understand hematophagous vector-host relationships. This study describes a customizable Next-Generation Sequencing (NGS) approach to identify blood meals from Rhodnius pallescens (Hemiptera: Reduviidae) triatomines using multiple barcoded primers and existing software to pick operational taxonomic units and match sequences for blood meal identification. We precisely identified all positive control samples using this method and further examined 74 wild-caught R. pallescens samples. With this novel blood meal identification method, we detected 13 vertebrate species in the blood meals, as well as single and multiple blood meals in individual bugs. Our results demonstrate the reliability and descriptive uses of our method.
准确鉴定血餐对于理解吸血媒介与宿主的关系至关重要。本研究描述了一种可定制的下一代测序（NGS）方法，该方法使用多个条形码引物和现有软件来挑选操作分类单元并匹配序列，以鉴定苍白猎蝽（半翅目：猎蝽科）吸食的血餐。我们使用这种方法精确鉴定了所有阳性对照样本，并进一步检测了74个野外捕获的苍白猎蝽样本。通过这种新型血餐鉴定方法，我们在血餐中检测到了13种脊椎动物物种，以及单个虫子体内的单重和多重血餐。我们的结果证明了我们方法的可靠性和描述性用途。
