Botanical Compound p-Anisaldehyde Repels Larval Lone Star Tick (Acari: Ixodidae), and Halts Reproduction by Gravid Adults.

The lone star tick, Amblyomma americanum (L.), widely distributed across eastern, southeastern, and midwestern regions of the United States and south into Mexico, is an obligate blood feeder that attaches to three hosts during the larval, nymphal, and adult stages. White-tailed deer and wild turkey are common hosts, as well as a wide variety of other avian and mammalian hosts. Amblyomma americanum is the most frequently reported species of tick to bite humans in the southeastern and southcentral United States, and it can transmit diseases that include erhlichiosis, rickettsiosis, tularemia, and protozoan infections. As A. americanum resistance to conventional insecticides becomes more common, alternative control tactics, such as application of bioactive botanical natural products are being investigated. p-Anisaldehyde has been found in many plant species and it has shown effects that include mortality, attractancy, and interference with host seeking. The series of bioassays we developed was effective for assessing a range of ixodid tick responses to chemicals. This first assessment of p-anisaldehyde on ticks focused mostly on larvae, usually the most vulnerable free-living stage, and on egg production by adults. Contact exposure to larvae resulted in an LD50 of 0.162% and an LD90 of 0.311% p-anisaldehyde. Although fumigation was not lethal to larval A. americanum, p-anisaldehyde was strongly repellent against them in several bioassays that indicate aspects of the repellency such as counteracting the negative geotaxic orientation on a vertical surface, the ability to trap larvae between and inside treatment barriers on different substrates and to divert upward larval movement from one surface to another, and the extent to which larvae fall from a treated surface. The compound at a relatively low concentration applied to gravid adults strongly reduces egg laying and the few eggs that are produced do not hatch. Aside from repelling larval A. americanum and halting reproduction, p-anisaldehyde has a variety of effects on other arthropods including attraction. Research on this compound as a potentially multifaceted pest management tool has been sparse. This study, for example, is the first to demonstrate p-anisaldehyde's repellent properties against an arthropod pest. p-Anisaldehyde might also act as a strong repellent against other tick species.