Liu Suqin, Pei Fenghua, Wang Xinhong, Li Deliang, Zhao Lixia, Song Yanyan, Chen Zhendong, Liu Bingrong
Department of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Translational Medicine Research and Cooperation Center of Northern China, Heilongjiang Academy of Medical Sciences, Heilongjiang, China.
Oncotarget. 2017 Mar 15;8(39):66528-66539. doi: 10.18632/oncotarget.16236. eCollection 2017 Sep 12.
This study was conducted to evaluate the immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute suppurative appendicitis and to determine whether TLR4/MYD88/NF-κB signaling pathway was activated in this process. 48 rabbits were assigned into 4 groups: group I, the mimic endoscopic retrograde appendicitis therapy group; group II, the appendectomy group; group III, the model group; and group IV, the blank group. White blood cells decreased, while levels of C-reactive protein, tumor necrosis factor-α, interleukin-6, interleukin-4, and interleukin-10 increased on the 2 day in group I and II. IgA in feces decreased at 2 weeks, while fecal microbiota changed at 2 and 4 weeks after appendectomy. CD8 cells in appendix of group I increased within 8 weeks. Upregulated expression of TLR4, MYD88, and nuclear NF-κB were detected on the 2 day in group I and II. Mimic endoscopic retrograde appendicitis therapy and appendectomy are effective ways for acute suppurative appendicitis. Mimic endoscopic retrograde appendicitis therapy was more preferable due to its advantage in maintaining intestinal immune function. TLR4/MYD88/NF-κB signaling pathway was activated in acute phase of appendicitis.
本研究旨在评估模拟内镜逆行性阑尾炎治疗和阑尾切除术对急性化脓性阑尾炎家兔的免疫影响，并确定在此过程中TLR4/MYD88/NF-κB信号通路是否被激活。48只家兔被分为4组：I组，模拟内镜逆行性阑尾炎治疗组；II组，阑尾切除组；III组，模型组；IV组，空白组。I组和II组在第2天时白细胞减少，而C反应蛋白、肿瘤坏死因子-α、白细胞介素-6、白细胞介素-4和白细胞介素-10水平升高。阑尾切除术后2周粪便中IgA减少，2周和4周时粪便微生物群发生变化。I组阑尾中的CD8细胞在8周内增加。I组和II组在第2天时检测到TLR4、MYD88和核NF-κB的表达上调。模拟内镜逆行性阑尾炎治疗和阑尾切除术是治疗急性化脓性阑尾炎的有效方法。模拟内镜逆行性阑尾炎治疗因其在维持肠道免疫功能方面的优势而更可取。TLR4/MYD88/NF-κB信号通路在阑尾炎急性期被激活。
