Newman Kayla D, Mhalhal Thaer R, Washington Martha C, Heath John C, Sayegh Ayman I
Gastroenterology Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL, 36088, USA.
Department of Anatomy and Histology, College of Veterinary Medicine, University of Basrah, Basrah, Iraq.
Dig Dis Sci. 2017 Dec;62(12):3350-3358. doi: 10.1007/s10620-017-4788-3. Epub 2017 Oct 13.
Peptide tyrosine tyrosine 3-36 (peptide YY 3-36 or PYY 3-36) reduces food intake by unknown site(s).
To test the hypothesis that the gastrointestinal tract contains sites of action regulating meal size (MS) and intermeal interval (IMI) length by PYY 3-36.
Peptide YY 3-36 (0, 1, 5, 10 and 20 nmol/kg) was injected in the aorta, the artery that supplies the gastrointestinal tract, prior to the onset of the dark cycle in free feeding male Sprague-Dawley rats and food intake was measured. Then, PYY 3-36 (25 nmol/kg) was injected intraperitoneally in these rats and Fos-like immunoreactivity (Fos-LI, a marker for neuronal activation) was quantified in the small intestinal enteric neurons, both myenteric and submucosal, and the dorsal vagal complex (DVC) of the hindbrain.
PYY 3-36 reduced first MS, decreased IMI length, shortened duration of first meal and increased Fos-LI in enteric and DVC neurons. However, PYY 3-36 failed to change the size of the second meal, satiety ratio, latency to first meal, number of meals and 24 h intake relative to saline control.
The gastrointestinal tract may contain sites of action regulating MS reduction by PYY 3-36.
肽YY 3 - 36（PYY 3 - 36）通过未知部位减少食物摄入量。
验证胃肠道含有通过PYY 3 - 36调节进餐量（MS）和餐间间隔（IMI）时长的作用位点这一假说。
在自由进食的雄性斯普拉格 - 道利大鼠黑暗周期开始前，将肽YY 3 - 36（0、1、5、10和20 nmol/kg）注入供应胃肠道的主动脉，并测量食物摄入量。然后，向这些大鼠腹腔注射PYY 3 - 36（25 nmol/kg），并对小肠肠神经元（肌间神经丛和黏膜下神经丛）以及后脑的迷走神经背核（DVC）中的Fos样免疫反应性（Fos - LI，神经元激活的标志物）进行定量分析。
PYY 3 - 36减少首次进餐量，缩短餐间间隔时长，缩短首次进餐持续时间，并增加肠神经元和DVC神经元中的Fos - LI。然而，相对于生理盐水对照，PYY 3 - 36未能改变第二次进餐量、饱腹感比率、首次进餐潜伏期、进餐次数和24小时摄入量。
胃肠道可能含有通过PYY 3 - 36调节进餐量减少的作用位点。
