通过耦合单细胞测量和计算机建模研究静止异质性。

Study Quiescence Heterogeneity by Coupling Single-Cell Measurements and Computer Modeling.

作者信息

Kwon Jungeun Sarah, Wang Xia, Yao Guang

机构信息

Department of Molecular and Cellular Biology, University of Arizona, 1007 E. Lowell Street, P.O. Box 210106, Tucson, AZ, USA.

出版信息

Methods Mol Biol. 2018;1686:287-299. doi: 10.1007/978-1-4939-7371-2_20.

DOI:10.1007/978-1-4939-7371-2_20

PMID:29030828

Abstract

Single-cell measurements combined with mathematical modeling and computer simulations are powerful tools for understanding and exploring dynamical behaviors of gene networks and cellular functions that they control. Here, we describe experimental and computational methods to study cellular quiescence and its heterogeneity at the single-cell level.

摘要

将单细胞测量与数学建模及计算机模拟相结合，是理解和探索基因网络及其所控制的细胞功能的动态行为的有力工具。在此，我们描述了在单细胞水平研究细胞静止及其异质性的实验和计算方法。