Wang Yuan, Feng Kewei, Liu Bin, Zhang Zhiwei, Wei Jianping, Yuan Yahong, Yue Tianli
Northwest A&F University, College of Food Science and Engineering, Yangling, Shaanxi, China.
Laboratory of Quality & Safety Risk Assessment for Agro-products (Yangling), Ministry of Agriculture, Yangling, Shaanxi, China.
J Sci Food Agric. 2018 May;98(7):2573-2581. doi: 10.1002/jsfa.8747. Epub 2017 Nov 28.
Fungal development in agricultural products may cause mycotoxin contamination, which is a significant threat to food safety. Patulin (PAT) and PAT-producer contamination has been established as a worldwide problem. The present study aimed to investigate the mycoflora and PAT-producers present in kiwifruits and environmental samples collected from orchards and processing plants in Shaanxi Province, China.
Variations in mycoflora were observed in different samples, with penicillia and aspergilli as the predominant genera. Approximately 42.86% of dropped fruits were contaminated with PAT-producers, which harbored the 6-methylsalicylic acid synthase and the isoepoxydon dehydrogenase genes that are involved in PAT biosynthesis. The growth of Penicillium expansum, Penicillium griseofulvum and Penicillium paneum in kiwi puree agar (KPA) medium and kiwi juice well fitted the modified Gompertz and Baranyi and Roberts models (R ≥ 0.95). A significant positive correlation between colony diameter and PAT content in KPA medium of P. expansum and P. griseofulvum was observed (P < 0.05).
The present study analyzed the mycofloral composition and the potential risk for PAT and PAT-producer contamination in kiwifruit, which may be utilized in the establishment of proper management practices in the kiwifruit industry. © 2017 Society of Chemical Industry.
农产品中的真菌生长可能导致霉菌毒素污染，这对食品安全构成重大威胁。展青霉素（PAT）及产PAT的污染物已被认定为一个全球性问题。本研究旨在调查中国陕西省果园和加工厂采集的猕猴桃及环境样本中的真菌区系和产PAT的菌株。
在不同样本中观察到真菌区系的差异，青霉属和曲霉属为主要属。约42.86%的落果被产PAT的菌株污染，这些菌株含有参与PAT生物合成的6-甲基水杨酸合酶和异环氧脱氧酮脱氢酶基因。扩展青霉、灰黄青霉和潘尼青霉在猕猴桃泥琼脂（KPA）培养基和猕猴桃汁中的生长很好地拟合了修正的Gompertz模型以及Baranyi和Roberts模型（R≥0.95）。观察到扩展青霉和灰黄青霉在KPA培养基中的菌落直径与PAT含量之间存在显著正相关（P＜0.05）。
本研究分析了猕猴桃中的真菌区系组成以及PAT和产PAT污染物的潜在风险，这可用于猕猴桃产业建立适当的管理措施。©2017化学工业协会。
