School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu Province 212013, China.
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu Province 212013, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Feb 15;191:172-179. doi: 10.1016/j.saa.2017.10.018. Epub 2017 Oct 9.
A simple relay identification fluorescence probe for Fe and phosphate anion with "on-off-on" switching was designed and synthesized based on the phenylthiazole and biphenylcarbonitrile. Probe 1 displayed highly selective and sensitive recognition to Fe in HEPES aqueous buffer (EtOH/HO=2:8, v/v, pH=7.4) solutions. The optimized structures and HOMO and LUMO of probe 1 and [1-Fe] complex were obtained by the density functional theory (DFT) calculations with B3LYP as the exchange and correlation functional using a suite of Gaussian 09 programs. The [1-Fe] complex solution also showed a high selectivity toward PO. The lower limits of detection of probe 1 to Fe and [1-Fe] complex to PO were estimated to 1.09×10M and 1.86×10M. Besides, the probe 1 also was used to detected the target ions in real water sample and living cells successfully.
基于苯并噻唑和联苯甲腈，设计并合成了一种简单的用于 Fe 和磷酸根阴离子的“开-关-开”切换的中继识别荧光探针。探针 1 在 HEPES 水性缓冲液（EtOH/HO=2:8，v/v，pH=7.4）溶液中对 Fe 表现出高度选择性和灵敏的识别。通过密度泛函理论（DFT）计算，使用高斯 09 程序套件，以 B3LYP 作为交换和相关功能，获得了探针 1 和[1-Fe]配合物的优化结构以及 HOMO 和 LUMO。[1-Fe]配合物溶液对 PO 也表现出高选择性。探针 1 对 Fe 和[1-Fe]配合物对 PO 的检测下限分别估计为 1.09×10M 和 1.86×10M。此外，探针 1 还成功用于检测实际水样和活细胞中的靶离子。
