Piao Meiling, Sun Lei, Zhang Qiangfeng Cliff
MOE Key Laboratory of Bioinformatics, Beijing Advanced Innovation Center for Structural Biology, Center for Synthetic and Systems Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
MOE Key Laboratory of Bioinformatics, Beijing Advanced Innovation Center for Structural Biology, Center for Synthetic and Systems Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Genomics Proteomics Bioinformatics. 2017 Oct;15(5):267-278. doi: 10.1016/j.gpb.2017.05.002. Epub 2017 Oct 12.
RNA folds into intricate structures that are crucial for its functions and regulations. To date, a multitude of approaches for probing structures of the whole transcriptome, i.e., RNA structuromes, have been developed. Applications of these approaches to different cell lines and tissues have generated a rich resource for the study of RNA structure-function relationships at a systems biology level. In this review, we first introduce the designs of these methods and their applications to study different RNA structuromes. We emphasize their technological differences especially their unique advantages and caveats. We then summarize the structural insights in RNA functions and regulations obtained from the studies of RNA structuromes. And finally, we propose potential directions for future improvements and studies.
RNA折叠成复杂的结构，这些结构对其功能和调控至关重要。迄今为止，已经开发出多种用于探测整个转录组结构（即RNA结构组）的方法。将这些方法应用于不同的细胞系和组织，为在系统生物学水平上研究RNA结构与功能的关系提供了丰富的资源。在本综述中，我们首先介绍这些方法的设计及其在研究不同RNA结构组中的应用。我们强调它们在技术上的差异，尤其是它们独特的优势和注意事项。然后，我们总结从RNA结构组研究中获得的关于RNA功能和调控的结构见解。最后，我们提出未来改进和研究的潜在方向。
