Hayakawa K, Hardy R R
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
J Exp Med. 1988 Nov 1;168(5):1825-38. doi: 10.1084/jem.168.5.1825.
We have used two monoclonal anti-murine T cell autoantibodies (SM3G11 and SM6C10) and multi-color immunofluorescence staining to resolve splenic CD4+ cells into four populations. Two of these populations (Fr. I and Fr. III, 35% and 10% of CD4+ cells) show mutually exclusive expression of these determinants and exhibit distinct functions. Fr. III secretes IL-4, but not IL-2 when activated by Con A, and includes memory T cells responsible for secondary antibody formation. In contrast, Fr. I secretes IL-2 but not IL-4 in response to Con A, and does not contribute to the secondary antibody response. Furthermore, these two fractions exhibit differential accessory cell dependence. Whereas Fr. III responds with B cells (and also non-B cells) as accessory cells in Con A-induced activation, Fr. I requires non-B cells. However, we found that many CD4+ cells (Fr. II, 40% of CD4+ cells) express both determinants and are not distinguishable with regard to lymphokine secretion, accessory cell effect, and memory T cell activity. Curiously, the fraction expressing neither determinant (Fr. IV, 10% of CD4+ cells) is unresponsive to experimental conditions used here. We discuss the possible relationships between these T cell subsets and the implications of differential expression of these determinants.
我们使用了两种抗小鼠T细胞单克隆自身抗体（SM3G11和SM6C10）以及多色免疫荧光染色，将脾脏CD4+细胞分为四个群体。其中两个群体（Fr. I和Fr. III，分别占CD4+细胞的35%和10%）显示出这些决定簇的相互排斥性表达，并表现出不同的功能。Fr. III在被刀豆蛋白A激活时分泌IL-4，但不分泌IL-2，并且包括负责二次抗体形成的记忆T细胞。相比之下，Fr. I在对刀豆蛋白A的反应中分泌IL-2但不分泌IL-4，并且对二次抗体反应没有贡献。此外，这两个部分表现出不同的辅助细胞依赖性。在刀豆蛋白A诱导的激活中，Fr. III以B细胞（以及非B细胞）作为辅助细胞做出反应，而Fr. I需要非B细胞。然而，我们发现许多CD4+细胞（Fr. II，占CD4+细胞的40%）同时表达这两种决定簇，并且在淋巴因子分泌、辅助细胞效应和记忆T细胞活性方面无法区分。奇怪的是，既不表达这两种决定簇的部分（Fr. IV，占CD4+细胞的10%）对这里使用的实验条件没有反应。我们讨论了这些T细胞亚群之间可能的关系以及这些决定簇差异表达的意义。
