抗原刺激后CD4+ T细胞的表型和功能改变。两种均分泌白细胞介素4的记忆性T细胞群体的解析。
Phenotypic and functional alteration of CD4+ T cells after antigen stimulation. Resolution of two populations of memory T cells that both secrete interleukin 4.
作者信息
Hayakawa K, Hardy R R
机构信息
Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
出版信息
J Exp Med. 1989 Jun 1;169(6):2245-50. doi: 10.1084/jem.169.6.2245.
Abstract
Phenotypic and functional alteration of murine CD4+ T cells after antigenic stimulation was studied using two anti-T cell mAbs recently described that define four distinct T cell subsets. Activation of T cells resulted in the permanent loss of 3G11 expression. However, two phenotypically distinct memory T cell populations were established depending on the system used; whereas those for anti-KLH antibody response were enriched in the fraction expression 6C10 (Fr. III), memory T cells for the allogeneic MLR lacked such expression (Fr. IV). Furthermore, successive stimulation with antigen in vitro resulted in secretion of IL-4 without detectable IL-2. This alteration of phenotype and interleukin secretion was also demonstrable when starting with 3G11+6C10- cells (Fr. I), the fraction that secretes IL-2 exclusively upon activation.
摘要
利用最近描述的两种抗T细胞单克隆抗体（mAbs）定义四个不同的T细胞亚群，研究了抗原刺激后小鼠CD4 + T细胞的表型和功能改变。T细胞的激活导致3G11表达的永久性丧失。然而，根据所使用的系统建立了两个表型不同的记忆T细胞群体；抗钥孔血蓝蛋白（KLH）抗体反应的记忆T细胞在表达6C10的部分（III组）中富集，而同种异体混合淋巴细胞反应（MLR）的记忆T细胞缺乏这种表达（IV组）。此外，体外连续用抗原刺激导致分泌白细胞介素-4（IL-4）而未检测到白细胞介素-2（IL-2）。当从3G11 + 6C10-细胞（I组）开始时，这种表型和白细胞介素分泌的改变也得到证实，该部分细胞仅在激活时分泌IL-2。
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