Department of Basic Sciences, School of Dentistry, São Paulo State University (Unesp), Rodovia Marechal Rondon km 527, CEP, Araçatuba, São Paulo, 16018-805, Brazil.
Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas - SBFis, School of Dentistry, São Paulo State University (Unesp), Araçatuba, São Paulo, Brazil.
Clin Oral Investig. 2018 Apr;22(3):1449-1461. doi: 10.1007/s00784-017-2249-1. Epub 2017 Oct 14.
The objective of this study was to investigate the effects of mate tea (MT) [Ilex paraguariensis] on alveolar socket healing after tooth extraction.
Sixteen male rats were divided into MT and control groups. MT was administered by intragastric gavage at a dose of 20 mg/kg/day for 28 days before and 28 days after right maxillary incisor extraction. The control group received an equal volume of water. Histopathological and histometric analysis of the neoformed bone area and osteocyte density were performed, as well as immunohistochemical analysis of osteocalcin (OCN), receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG), tartrate-resistant acid phosphatase (TRAP), and manganese superoxide dismutase (MnSOD) in the alveolar socket. Calcium, phosphorus, alkaline phosphatase (ALP) activity, total antioxidant capacity (TAC), and malondialdehyde (MDA) were measured in plasma, whereas TRAP activity was determined in serum.
Histometry evidenced an increase in bone area (P < 0.0001) and osteocyte density (P < 0.0001). MT increased immunolabeling of MnSOD (P < 0.001), OCN (P < 0.0001), RANKL (P < 0.001), OPG (P < 0.0001), and TRAP (P < 0.001). Calcium and phosphorus concentrations did not differ between the groups. In addition, MT enhanced ALP (P < 0.05) and TRAP (P < 0.0001) activities. MT increased the TAC (P < 0.001), whereas it reduced MDA concentrations (P < 0.0001).
MT increases bone area and osteocyte density in the alveolar socket healing on day 28 after tooth extraction.
Regular MT ingestion improves the antioxidant defenses and bone formation, which is beneficial for alveolar socket bone healing after tooth extraction.
本研究旨在探讨马黛茶（Mate tea，MT）[巴拉圭冬青（Ilex paraguariensis）]对拔牙后牙槽骨愈合的影响。
16 只雄性大鼠随机分为 MT 组和对照组。MT 组在右侧上颌切牙拔除前 28 天和拔除后 28 天内每天通过灌胃给予 20mg/kg 的剂量，对照组给予等体积的水。对新形成的骨区域和骨细胞密度进行组织病理学和组织计量学分析，并对牙槽骨中的骨钙素（Osteocalcin，OCN）、核因子 kappa-B 受体激活剂配体（Receptor activator of nuclear factor kappa-B ligand，RANKL）、骨保护素（Osteoprotegerin，OPG）、抗酒石酸酸性磷酸酶（Tartrate-resistant acid phosphatase，TRAP）和锰超氧化物歧化酶（Manganese superoxide dismutase，MnSOD）进行免疫组织化学分析。测定血浆中的钙、磷、碱性磷酸酶（Alkaline phosphatase，ALP）活性、总抗氧化能力（Total antioxidant capacity，TAC）和丙二醛（Malondialdehyde，MDA），血清中的 TRAP 活性。
组织计量学证据表明，骨面积增加（P<0.0001）和骨细胞密度增加（P<0.0001）。MT 增加了 MnSOD（P<0.001）、OCN（P<0.0001）、RANKL（P<0.001）、OPG（P<0.0001）和 TRAP（P<0.001）的免疫标记。两组间钙、磷浓度无差异。此外，MT 增强了 ALP（P<0.05）和 TRAP（P<0.0001）的活性。MT 增加了 TAC（P<0.001），同时降低了 MDA 浓度（P<0.0001）。
MT 可增加拔牙后第 28 天牙槽骨愈合中的骨面积和骨细胞密度。
规律摄入 MT 可增强抗氧化防御能力和骨形成，这有利于拔牙后牙槽骨的愈合。
