Pereira Ariana Aparecida Ferreira, Tirapeli Keny Gonçalves, Chaves-Neto Antonio Hernandes, da Silva Brasilino Matheus, da Rocha Cláudia Quintino, Belló-Klein Adriane, Llesuy Suzana Francisca, Dornelles Rita Cássia Menegati, Nakamune Ana Cláudia de Melo Stevanato
Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas, SBFis/UNESP, Univ Estadual Paulista, Araçatuba, São Paulo, Brazil; Laboratory of Biochemistry, Department of Basic Sciences, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Araçatuba, São Paulo, Brazil.
Laboratory of Biochemistry, Department of Basic Sciences, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Araçatuba, São Paulo, Brazil.
Exp Gerontol. 2017 Apr;90:14-18. doi: 10.1016/j.exger.2017.01.011. Epub 2017 Jan 14.
Perimenopause is a period in a woman's life that precedes menopause and is characterized by hormonal changes that result in increased oxidative stress. Since oxidative stress is associated with age-related diseases and perimenopausal symptoms including somato-vegetative manifestations, nutritional antioxidant supplementation may be an effective approach to minimizing this stress. Mate tea (MT) (Ilex paraguariensis), a typical and inexpensive beverage consumed in the Brazilian south-east, Argentina and Uruguay, increases antioxidant defense. We hypothesized that MT could minimize oxidative stress during perimenopause by modulating enzymatic antioxidant defense. To test this, we analyzed the lipid oxidative damage and antioxidant defense in erythrocytes and liver of rats, after MT treatment. Female Wistar rats (aged 16months) in proven perimenopause period received 20mg/kgBW/day of mate tea, by gavage (PM+MT group) or water (PM group). Female rats aged 4months (AD group) received water. Erythrocytes and liver were used to determine lipid oxidative damage, determined by malondialdehyde (MDA); superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities. Total plasma antioxidant capacity was examined by ferric reducing antioxidant power assay (FRAP) and estrogen by radioimmunoassay. MT increased FRAP and did not change estrogen levels. Increased SOD and GPx, and reduced MDA were observed in both tissues studied. Increased CAT activity was observed only in the liver. We confirmed the hypothesis that MT was capable of minimizing oxidative stress in this period of life by modulating antioxidant defense.
围绝经期是女性绝经前的一段时期，其特征是激素变化导致氧化应激增加。由于氧化应激与年龄相关疾病以及包括躯体植物神经症状在内的围绝经期症状有关，补充营养抗氧化剂可能是减轻这种应激的有效方法。马黛茶（MT）（巴拉圭冬青）是巴西东南部、阿根廷和乌拉圭消费的一种典型且价格低廉的饮品，可增强抗氧化防御能力。我们假设MT可通过调节酶促抗氧化防御来减轻围绝经期的氧化应激。为验证这一点，我们在MT处理后分析了大鼠红细胞和肝脏中的脂质氧化损伤及抗氧化防御情况。处于围绝经期的16月龄雌性Wistar大鼠通过灌胃接受20mg/kg体重/天的马黛茶（围绝经期+MT组）或水（围绝经期组）。4月龄雌性大鼠（成年组）接受水。通过丙二醛（MDA）测定红细胞和肝脏中的脂质氧化损伤；测定超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）的活性。通过铁还原抗氧化能力测定法（FRAP）检测血浆总抗氧化能力，并通过放射免疫分析法检测雌激素。MT提高了FRAP且未改变雌激素水平。在所研究的两种组织中均观察到SOD和GPx增加，MDA减少。仅在肝脏中观察到CAT活性增加。我们证实了MT能够通过调节抗氧化防御来减轻这一生命阶段氧化应激的假设。
