Mizumoto K, Sugawara I, Ito W, Kodama T, Hayami M, Mori S
Division of Biochemical Genetics, Meiji Institute of Health Science, Kanagawa, Japan.
Jpn J Exp Med. 1988 Jun;58(3):145-51.
We reported previously that homopolysaccharides with sulfate groups revealed immunomodulating activities--lymphocyte mitogens. We further investigated the role of homopolysaccharides in a different system--cultivation of Molt-4. clone no.8 with supernatant from human T cell lymphotropic virus type III (HTLV-III)-infected TALL-1, utilizing cytopathic effects (CPE), fluorescence antibody technique (FAT), reverse transcriptase (RT) assay and cell proliferation assay. Sulfated homopolysaccharides such as fucoidan, dextran sulfate with three different molecular weights, cellulose sulfate and k-carrageenan showed most potent anti-HTLV-III activities at mitogenic doses. However, neutral homopolysaccharides had no effects on anti-HTLV-III activities Sulfated heteropolysaccharides such a heparin and heparan sulfate had a little effect on anti-HTLV-III activities. It is suggested that sulfate group is most important in inhibiting growth of HTLV-III, but the structure of the polysaccharides is also important, because homopolysaccharides are more potent anti-HTLV-III agents than heteropolysaccharides.
我们之前报道过，带有硫酸基团的同多糖具有免疫调节活性——淋巴细胞有丝分裂原。我们进一步在不同系统中研究了同多糖的作用——利用细胞病变效应（CPE）、荧光抗体技术（FAT）、逆转录酶（RT）测定法和细胞增殖测定法，用人III型嗜T细胞病毒（HTLV-III）感染的TALL-1的上清液培养Molt-4克隆8号。硫酸化同多糖，如岩藻依聚糖、三种不同分子量的硫酸葡聚糖、硫酸纤维素和κ-卡拉胶，在促有丝分裂剂量下显示出最有效的抗HTLV-III活性。然而，中性同多糖对抗HTLV-III活性没有影响。硫酸化杂多糖，如肝素和硫酸乙酰肝素，对抗HTLV-III活性有一点影响。提示硫酸基团在抑制HTLV-III生长中最为重要，但多糖的结构也很重要，因为同多糖比杂多糖是更有效的抗HTLV-III药物。
