Rouzer Siara K, Cole Jesse M, Johnson Julia M, Varlinskaya Elena I, Diaz Marvin R
Department of Psychology, Center for Development and Behavioral Neuroscience, Developmental Exposure Alcohol Research Center, Binghamton University, Binghamton, NY, United States.
Front Behav Neurosci. 2017 Sep 29;11:183. doi: 10.3389/fnbeh.2017.00183. eCollection 2017.
Among the numerous consequences of prenatal alcohol exposure (PAE) is an increase in anxiety-like behavior that can prove debilitating to daily functioning. A significant body of literature has linked gestational day 12 (G12) heavy ethanol exposure with social anxiety, evident in adolescent males and females. However, the association between non-social anxiety-like behavior and moderate alcohol exposure, a more common pattern of drinking in pregnant women, is yet unidentified. To model moderate PAE (mPAE), we exposed pregnant Sprague-Dawley rats to either room air or vaporized ethanol for 6 h on G12. Adolescent offspring were then tested on postnatal days (P) 41-47 in one of the following four anxiety assays: novelty-induced hypophagia (NIH), elevated plus maze (EPM), light-dark box (LDB) and open-field (OF). Our findings revealed significant increases in measures of anxiety-like behavior in male PAE offspring in the NIH, LDB and OF, with no differences observed in females on any test. Additionally, male offspring who demonstrated heightened anxiety-like behavior as adolescents demonstrated decreased anxiety-like behavior in adulthood, as measured by a marble-burying test (MBT), while females continued to be unaffected in adulthood. These results suggest that mPAE leads to dynamic changes in anxiety-like behavior exclusively in male offspring.
产前酒精暴露（PAE）的众多后果之一是焦虑样行为增加，这可能会对日常功能造成损害。大量文献将妊娠第12天（G12）重度乙醇暴露与社交焦虑联系起来，这在青春期男性和女性中都很明显。然而，非社交焦虑样行为与中度酒精暴露（孕妇中更常见的饮酒模式）之间的关联尚未明确。为了模拟中度PAE（mPAE），我们在G12将怀孕的斯普拉格-道利大鼠暴露于室内空气或汽化乙醇中6小时。然后在出生后第41 - 47天对青春期后代进行以下四种焦虑测试之一：新奇诱导性摄食减少（NIH）、高架十字迷宫（EPM）、明暗箱（LDB）和旷场试验（OF）。我们的研究结果显示，在NIH、LDB和OF测试中，雄性PAE后代的焦虑样行为指标显著增加，而在任何测试中雌性均未观察到差异。此外，通过埋珠试验（MBT）测量，在青春期表现出焦虑样行为增强的雄性后代在成年期表现出焦虑样行为减少，而雌性在成年期继续未受影响。这些结果表明，mPAE仅导致雄性后代焦虑样行为的动态变化。
