The Empirical Distribution of Singletons for Geographic Samples of DNA Sequences.

Rare variants are important for drawing inference about past demographic events in a species history. A singleton is a rare variant for which genetic variation is carried by a unique chromosome in a sample. How singletons are distributed across geographic space provides a local measure of genetic diversity that can be measured at the individual level. Here, we define the empirical distribution of singletons in a sample of chromosomes as the proportion of the total number of singletons that each chromosome carries, and we present a theoretical background for studying this distribution. Next, we use computer simulations to evaluate the potential for the empirical distribution of singletons to provide a description of genetic diversity across geographic space. In a Bayesian framework, we show that the empirical distribution of singletons leads to accurate estimates of the geographic origin of range expansions. We apply the Bayesian approach to estimating the origin of the cultivated plant species . (pearl millet) in Africa, and find support for range expansion having started from Northern Mali. Overall, we report that the empirical distribution of singletons is a useful measure to analyze results of sequencing projects based on large scale sampling of individuals across geographic space.