Faculdade de Medicina Veterinária, Centro Colaborador de Defesa Agropecuária do Brasil Central, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brasil.
Centro Nacional de Pesquisa em Gado de Corte, Empresa Brasileira de Pesquisa Agropecuária, Campo Grande, Mato Grosso do Sul, Brasil.
Transbound Emerg Dis. 2018 Apr;65(2):476-479. doi: 10.1111/tbed.12727. Epub 2017 Oct 16.
A total of 27 seminal plasma samples from cattle-breeding farms or semen centres located in Minas Gerais, Brazil, previously negative by serological and tested positive for Brucella spp. with primer specific for the amplification of the gene virb5 by polymerase chain reaction (PCR) were analysed for the detection of Brucella abortus DNA by PCR. It was found that nine samples (33.33%) contained B. abortus B19 strain DNA, two (7.40%) contained B. abortus DNA and five (18.51%) contained both DNA. The larger number of samples with B. abortus B19 strain DNA would explained by the environmental contamination by vaccinated females with persistent excretion or some illegal vaccination process. It is first reported of male bovines detected with both DNA.
总共对来自巴西米纳斯吉拉斯州的奶牛养殖场或精液中心的 27 个精液样本进行了分析，这些样本先前通过血清学检测呈阴性，但用针对聚合酶链反应 (PCR) 扩增 virb5 基因的引物检测 Brucella spp. 呈阳性。通过 PCR 检测 Brucella abortus DNA。结果发现，有 9 个样本（33.33%）含有 B. abortus B19 株 DNA，2 个样本（7.40%）含有 B. abortus DNA，5 个样本（18.51%）同时含有两种 DNA。B. abortus B19 株 DNA 样本数量较多的原因可能是接种疫苗的雌性动物持续排泄或某些非法接种过程造成了环境污染。这是首次报道同时检测到两种 DNA 的雄性牛。
