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应用聚合酶链反应检测血清阴性公牛精液中的布鲁氏菌属 DNA。

Detection of Brucella spp. DNA in the semen of seronegative bulls by polymerase chain reaction.

机构信息

Faculty of Veterinary Medicine, Collaborating Centre of Agricultural Protection in Central Brazil, Federal University of Uberlândia, Uberlândia, MG, Brazil.

出版信息

Transbound Emerg Dis. 2013 Aug;60(4):376-7. doi: 10.1111/j.1865-1682.2012.01347.x. Epub 2012 Jun 6.

DOI:10.1111/j.1865-1682.2012.01347.x
PMID:22672525
Abstract

Semen samples from 88 reproductively mature bulls were screened to detect the presence of Brucella spp. by polymerase chain reaction. Twenty-seven samples were found to be positive, underscoring the importance of researching brucellosis in males and the need for greater care in the selection of sperm-donating bulls for semen centres.

摘要

对 88 头生殖成熟的公牛的精液样本进行了筛查,通过聚合酶链反应检测布鲁氏菌属的存在。发现有 27 个样本呈阳性,这强调了研究雄性布鲁氏菌病的重要性,也需要在选择精液中心的捐精公牛时更加小心。

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