Massilia neuiana sp. nov., isolated from wet soil.

A novel aerobic, Gram-stain-negative, rod-shaped, motile bacterium, strain PTW21, was isolated from wet soil. 16S rRNA gene sequence phylogenetic analysis of strain PTW21 revealed an affiliation to the genus Massilia and it shared 98.5 and 98.1 % similarity with Massilianiastensis 5516 S-1 and Massilia tieshanensis TS3, respectively. Growth occurred at 10-45 °C, pH 4.5-12.5 and NaCl concentrations up to 2 % (w/v). The major fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C16 : 0. The predominant respiratory quinone was Q-8. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain PTW21 was 64.6 mol%. The results of DNA-DNA hybridization revealed that strain PTW21 showed 37.4 % relatedness with Massilia niastensis 5516 S-1 and 40.0 % with M. tieshanensis TS3. Based on phenotypic, chemotaxonomic and phylogenetic differences, strain PTW21 (=CICC 24113=BCRC 81061) is proposed as the type strain of novel species of the genus Massilia with the names Massilia neuiana sp. nov.