Zhao Xin, Li Xuejie, Qi Nan, Gan Meijun, Pan Yujin, Han Tianfang, Hu Xiaomin
Department of Environmental Engineering, School of Resource and Civil Engineering, Northeastern University, Shenyang 110004, PR China.
Int J Syst Evol Microbiol. 2017 Dec;67(12):4943-4947. doi: 10.1099/ijsem.0.002333. Epub 2017 Oct 16.
A novel aerobic, Gram-stain-negative, rod-shaped, motile bacterium, strain PTW21, was isolated from wet soil. 16S rRNA gene sequence phylogenetic analysis of strain PTW21 revealed an affiliation to the genus Massilia and it shared 98.5 and 98.1 % similarity with Massilianiastensis 5516 S-1 and Massilia tieshanensis TS3, respectively. Growth occurred at 10-45 °C, pH 4.5-12.5 and NaCl concentrations up to 2 % (w/v). The major fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C16 : 0. The predominant respiratory quinone was Q-8. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain PTW21 was 64.6 mol%. The results of DNA-DNA hybridization revealed that strain PTW21 showed 37.4 % relatedness with Massilia niastensis 5516 S-1 and 40.0 % with M. tieshanensis TS3. Based on phenotypic, chemotaxonomic and phylogenetic differences, strain PTW21 (=CICC 24113=BCRC 81061) is proposed as the type strain of novel species of the genus Massilia with the names Massilia neuiana sp. nov.
从潮湿土壤中分离出一株新型需氧、革兰氏染色阴性、杆状、具运动性的细菌，菌株PTW21。对菌株PTW21的16S rRNA基因序列进行系统发育分析表明，它隶属于马赛菌属，与纳氏马赛菌5516 S-1和铁山马赛菌TS3的相似性分别为98.5%和98.1%。该菌在10 - 45°C、pH 4.5 - 12.5以及NaCl浓度高达2%（w/v）的条件下均可生长。主要脂肪酸为总和特征3（C16 : 1ω7c和/或异-C15 : 0 2-OH）和C16 : 0。主要呼吸醌为Q-8。主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油。菌株PTW21的DNA G+C含量为64.6 mol%。DNA-DNA杂交结果显示，菌株PTW21与纳氏马赛菌5516 S-1的相关性为37.4%，与铁山马赛菌TS3的相关性为40.0%。基于表型、化学分类和系统发育差异，菌株PTW21（=CICC 24113=BCRC 81061）被提议作为马赛菌属一个新物种的模式菌株，命名为新纳氏马赛菌（Massilia neuiana sp. nov.）
