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人类Ⅱ类主要组织相容性基因的保守上游序列增强了野生型而非突变型B细胞系中Ⅱ类基因的表达。

Conserved upstream sequences of human class II major histocompatibility genes enhance expression of class II genes in wild-type but not mutant B-cell lines.

作者信息

Sloan J H, Boss J M

机构信息

Department of Microbiology and Immunology, Emory University, Atlanta, GA.

出版信息

Proc Natl Acad Sci U S A. 1988 Nov;85(21):8186-90. doi: 10.1073/pnas.85.21.8186.

DOI:10.1073/pnas.85.21.8186
PMID:2903501
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC282392/
Abstract

Class II major histocompatibility genes contain a conserved upstream sequence (CUS) that is important in the expression of these genes. This region has been divided into two major elements, the X box and the Y box. The ability of these elements to mediate transcription of a heterologous promoter was assayed upon transfection into a B-cell line (Raji), a class II-specific trans-acting factor-deficient B-cell line (RJ2.2.5 cells), and a T-cell line (Jurkat). The results showed that the X box element was responsible for directing tissue-specific expression when Raji cells were compared to Jurkat cells. The X box could not direct expression of the heterologous promoter in the trans-acting factor-deficient cell line, indicating that the X box is an ultimate target of the missing or defective factor in the RJ2.2.5 cell line. The Y box directed an equal but extremely low level of transcription in this system in both the mutant and wild-type B-cell lines, suggesting that this element is not involved in B-cell expression or as a target of the mutant factor.

摘要

II类主要组织相容性基因包含一个保守的上游序列（CUS），该序列对这些基因的表达很重要。该区域已被分为两个主要元件，即X框和Y框。将这些元件转染到B细胞系（拉吉细胞）、II类特异性反式作用因子缺陷的B细胞系（RJ2.2.5细胞）和T细胞系（ Jurkat细胞）中，检测它们介导异源启动子转录的能力。结果表明，与Jurkat细胞相比，当拉吉细胞时，X框元件负责指导组织特异性表达。X框不能在反式作用因子缺陷的细胞系中指导异源启动子的表达，这表明X框是RJ2.2.5细胞系中缺失或缺陷因子的最终靶点。在该系统中，Y框在突变型和野生型B细胞系中均指导相同但极低水平的转录，这表明该元件不参与B细胞表达，也不是突变因子的靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca4f/282392/06564c988edf/pnas00300-0368-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca4f/282392/518da061786b/pnas00300-0367-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca4f/282392/8bb12fc5d253/pnas00300-0368-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca4f/282392/06564c988edf/pnas00300-0368-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca4f/282392/518da061786b/pnas00300-0367-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca4f/282392/8bb12fc5d253/pnas00300-0368-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca4f/282392/06564c988edf/pnas00300-0368-b.jpg

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本文引用的文献

1
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J Exp Med. 1980 Dec 1;152(6):1709-19. doi: 10.1084/jem.152.6.1709.
2
Ia-negative B-cell variants reveal a coordinate regulation in the transcription of the HLA class II gene family.Ia阴性B细胞变体揭示了HLA II类基因家族转录中的协同调控。
Immunogenetics. 1984;19(4):349-53. doi: 10.1007/BF00345408.
3
Immune interferon activates multiple class II major histocompatibility complex genes and the associated invariant chain gene in human endothelial cells and dermal fibroblasts.
转录因子NF-YA和NF-YB被鉴定为与主要组织相容性复合体II类基因I-Aβ启动子结合的因子A和因子B。
Biochem J. 1996 Aug 1;317 ( Pt 3)(Pt 3):771-7. doi: 10.1042/bj3170771.
4
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5
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Nucleic Acids Res. 1993 Dec 25;21(25):6010-9. doi: 10.1093/nar/21.25.6010.
6
Affinity enrichment and functional characterization of TRAX1, a novel transcription activator and X1-sequence-binding protein of HLA-DRA.TRAX1的亲和富集与功能表征，TRAX1是一种新型转录激活因子及HLA - DRA的X1序列结合蛋白。
Mol Cell Biol. 1995 Jan;15(1):282-9. doi: 10.1128/MCB.15.1.282.
7
Physiologic target of the Air-1 trans-activator revealed by stable transfection assay.通过稳定转染试验揭示的Air-1反式激活因子的生理靶点。
Immunogenetics. 1994;39(1):8-14. doi: 10.1007/BF00171791.
8
Effect of the AIR-1 locus on the activation of an enhancerless HLA-DQA1 promoter.
Immunogenetics. 1990;31(5-6):368-76. doi: 10.1007/BF02115012.
9
Loss of a DNA-protein complex correlates with extinguished major histocompatibility complex class II expression in a human B cell.一种DNA-蛋白质复合物的缺失与人类B细胞中主要组织相容性复合体II类表达的消失相关。
J Exp Med. 1990 Jun 1;171(6):2159-64. doi: 10.1084/jem.171.6.2159.
10
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4
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Nature. 1981 Nov 19;294(5838):228-32. doi: 10.1038/294228a0.
9
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Proc Natl Acad Sci U S A. 1984 Aug;81(16):5199-203. doi: 10.1073/pnas.81.16.5199.
10
cis and trans activation of globin gene transcription in transient assays.在瞬时分析中珠蛋白基因转录的顺式和反式激活
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7428-32. doi: 10.1073/pnas.80.24.7428.