a Institute of Biotechnology and Genetic Engineering , The University of Agriculture Peshawar , Peshawar , Pakistan.
Anim Biotechnol. 2018 Jul 3;29(3):216-226. doi: 10.1080/10495398.2017.1366340. Epub 2017 Oct 16.
Pakistan is rich in sheep genetic resources. Balkhi, Hashtnagri, and Michni are neighboring sheep populations found in Khyber Pakhtunkhwa province of Pakistan. In this study, we analyzed the genetic structures and bottleneck incidents within these sheep populations using 31 microsatellite DNA markers. Total numbers of 116, 100, and 95 alleles, with average numbers of 3.20, 3.26, and 3.74 alleles per locus were observed, respectively, in Balkhi, Hashtnagri, and Michni population. Mean observed heterozygosity was 0.402 in Balkhi, 0.416 in Hashtnagri, and 0.522 in Michni population. All the three sheep populations showed significantly high inbreeding. Michni population was found to be in mutation drift equilibrium, showing the absence of genetic bottleneck. The data of Balkhi and Hashtnagri indicated the presence of genetic bottleneck in these populations. These results suggest a moderate level of genetic diversity within Michni population that may be useful for breed improvement programs. Hashtnagri and Balkhi populations having low within breed genetic variability may contain some valuable characteristics that need to be conserved.
巴基斯坦拥有丰富的绵羊遗传资源。巴尔基、哈什塔格纳里和米希尼是巴基斯坦开伯尔-普赫图赫瓦省的毗邻绵羊群体。在这项研究中，我们使用 31 个微卫星 DNA 标记分析了这些绵羊群体的遗传结构和瓶颈事件。在巴尔基、哈什塔格纳里和米希尼群体中，分别观察到 116、100 和 95 个等位基因，平均每个位点 3.20、3.26 和 3.74 个等位基因。巴尔基群体的平均观察杂合度为 0.402，哈什塔格纳里群体为 0.416，米希尼群体为 0.522。三个绵羊群体都表现出显著的近交。米希尼群体处于突变漂移平衡状态，表明不存在遗传瓶颈。巴尔基和哈什塔格纳里群体的数据表明，这些群体存在遗传瓶颈。这些结果表明，米希尼群体具有中等水平的遗传多样性，可能对品种改良计划有用。哈什塔格纳里和巴尔基群体的品种内遗传变异较低，可能包含一些需要保护的有价值的特征。
