a Animal Genome Research Unit, Agrogenomics Research Center, National Institute of Agrobiological Sciences , Tsukuba , Ibaraki , Japan.
b Office of Evaluation, National Agriculture and Food Research Organization , Tsukuba , Ibaraki , Japan.
Anim Biotechnol. 2018 Jul 3;29(3):227-233. doi: 10.1080/10495398.2017.1367691. Epub 2017 Oct 16.
Widely used antipig CD4 monoclonal antibodies (mAbs) fail to recognize CD4 alleles characteristic of miniature pig lines such as the National Institutes of Health (NIH) miniature pigs and microminipigs. We surveyed polymorphisms in the coding sequence of the porcine CD4 gene among Western and Oriental pig breeds and Japanese wild boars and investigated their distribution. Of the 13 alleles that we identified among the 47 animals, 2 in group I and 3 in group II were found exclusively in Western breed pigs. Group IV alleles, which included mAb-nonbinding alleles, were found frequently in Oriental breed pigs, suggesting that the mAb-nonbinding allele arose from the gene pool of Oriental pigs. Group IV alleles were also found in Duroc and Large White pigs, suggesting genetic inflow from Oriental pig breeds into Western breeds. Comparison of the CD4 sequences of species in Cetartiodactyla suggested that the group IV alleles in Sus scrofa occurred before the divergence of this species from the other artiodactyls. The different antibody specificities of the various CD4 alleles may facilitate the discrimination of T-cell populations in transplantation studies using miniature pigs. The significance of the preservation of CD4 polymorphisms to immune function in pigs warrants further investigation.
广泛使用的抗猪 CD4 单克隆抗体 (mAb) 无法识别 NIH 小型猪和微型猪等小型猪系特有的 CD4 等位基因。我们调查了西方和东方猪品种以及日本野猪中猪 CD4 基因编码序列的多态性，并研究了它们的分布。在 47 只动物中，我们鉴定了 13 种等位基因，其中 I 组 2 种和 II 组 3 种仅存在于西方品种猪中。IV 组等位基因，包括 mAb 不结合的等位基因，在东方品种猪中频繁发现，表明 mAb 不结合的等位基因来自东方猪品种的基因库。IV 组等位基因也存在于杜洛克和长白猪中，表明东方猪品种的基因流入了西方品种。对偶蹄目物种的 CD4 序列进行比较表明，Sus scrofa 中的 IV 组等位基因出现在该物种与其他偶蹄目动物分化之前。不同 CD4 等位基因的不同抗体特异性可能有助于在使用小型猪的移植研究中区分 T 细胞群体。保存猪 CD4 多态性对免疫功能的意义值得进一步研究。
