紫檀芪对鼠源前成骨细胞 MC3T3-E1 增殖、分化及矿化的促进作用。
Promoting Effect of Pinostrobin on the Proliferation, Differentiation, and Mineralization of Murine Pre-osteoblastic MC3T3-E1 Cells.
机构信息
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Life Science and Biotechnique Research Center, Northeast Agricultural University, Harbin 150030, China.
出版信息
Molecules. 2017 Oct 16;22(10):1735. doi: 10.3390/molecules22101735.
Pinostrobin (PI), a natural flavonoid found in a variety of plants, is well known for its rich pharmacological activities. However, its osteogenic function remains unclear. The aim of this study is to evaluate the effect of PI on the proliferation, differentiation, and mineralization of murine pre-osteoblastic MC3T3-E1 cells in vitro using MTT, alkaline phosphatase (ALP) activity, the synthesis of collagen I (Col I) assay, and Von-Kossa staining, respectively. The expression of osteocalcin (OCN) mRNA in cells was detected by real-time PCR. The effect of PI on the differentiation of dexamethasone (DEX)-suppressed cells was also investigated. The results showed that PI greatly promoted the proliferation of MC3T3-E1 cells at 5-80 μg/mL ( < 0.05 or < 0.01), and caused a significant elevation of ALP activity, Col I content, and mineralization of osteoblasts at 10-40 μg/mL ( < 0.05 or < 0.01), and the expression levels of OCN gene were greatly upregulated after PI treatment ( < 0.01). Furthermore, PI could rescue the inhibition effect of cell differentiation induced by DEX. Taken together, these results indicated that PI could directly promote proliferation, differentiation, and mineralization of MC3T3-E1 cells and has potential for use as a natural treatment for osteoporosis.
表儿茶素（PI）是一种天然黄酮类化合物，广泛存在于多种植物中，具有丰富的药理活性。然而，其成骨功能尚不清楚。本研究旨在通过 MTT、碱性磷酸酶（ALP）活性、胶原蛋白 I（Col I）合成测定和 Von-Kossa 染色分别评估 PI 对体外小鼠前成骨细胞 MC3T3-E1 细胞增殖、分化和矿化的影响。采用实时 PCR 检测细胞中骨钙素（OCN）mRNA 的表达。还研究了 PI 对地塞米松（DEX）抑制细胞分化的影响。结果表明，PI 在 5-80μg/mL 范围内（<0.05 或 <0.01）可显著促进 MC3T3-E1 细胞的增殖，在 10-40μg/mL 范围内（<0.05 或 <0.01）可显著提高 ALP 活性、Col I 含量和成骨细胞矿化，PI 处理后 OCN 基因的表达水平也显著上调（<0.01）。此外，PI 可挽救 DEX 诱导的细胞分化抑制作用。综上所述，这些结果表明 PI 可直接促进 MC3T3-E1 细胞的增殖、分化和矿化，具有作为天然骨质疏松治疗药物的潜力。
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