State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 100050, China.
J Nat Prod. 2017 Oct 27;80(10):2609-2614. doi: 10.1021/acs.jnatprod.7b00054. Epub 2017 Oct 16.
From the cultured endophytic fungus Glomerella cingulata isolated from a toxic plant, Gelsemium elegans, one new phenanthrene (1), four new sesquiterpenes (2-5), and three known sesquiterpenes (6-8) were isolated. Their structures were elucidated using spectroscopic methods. Based on the ECD calculations, the absolute configurations of the new compounds were determined. Compounds 2, 4, and 5 inhibited lipopolysaccharide (LPS)-induced NO production in BV2 cells by 50.6, 36.1, and 29.4%, respectively, at 1 μM (positive control curcumin, IC = 4.0 μM).
从有毒植物钩吻中分离到的内生真菌球腔菌（Glomerella cingulata）中分离得到一个新的菲（1），四个新的倍半萜（2-5）和三个已知的倍半萜（6-8）。通过光谱方法阐明了它们的结构。基于ECD 计算，确定了新化合物的绝对构型。在 1 μM 时，化合物 2、4 和 5 分别抑制 LPS 诱导的 BV2 细胞中 NO 的产生 50.6%、36.1%和 29.4%（阳性对照姜黄素，IC = 4.0 μM）。
