Chen Chih-Yu, Tsai Teh-Hua, Wu Pei-Ssu, Tsao Shuo-En, Huang Yu-Shan, Chung Ying-Chien
a Department of Tourism and Leisure , Hsing Wu University , Taipei , Taiwan.
b Department of Chemical Engineering and Biotechnology , National Taipei University of Technology , Taipei , Taiwan.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2018 Jan 28;53(2):108-115. doi: 10.1080/10934529.2017.1377580. Epub 2017 Oct 16.
This study was conducted to select electrogenic bacteria from wastewater sludge. Phylogenetic analysis revealed that Proteobacteria was the dominant phylum in the microbial fuel cell (MFC) during the decomposition process of organic pollutants. Five culturable bacteria strains - namely, Bacillus subtilis, Flavobacterium sp., Aeromonas hydrophila, Citrobacter freundii, and Stenotrophomonas sp. - have a double potential in dye removal and electricity generation. We inoculated the mixed electrogenic bacteria at a specific ratio and treated them with a triphenylmethane dye, Victoria blue R (VBR), to evaluate their electricity generation ability for the artificial and real wastewater. The results of the VBR shock-loading experiment indicated that the inoculated MFC could adapt to shock loading in 1-2 days and exhibited high removal efficiency (95-100%) for 100-800 mg L VBR with a power density of 8.62 ± 0.10 to 34.81 ± 0.25 mW m. The selected electrogenic bacteria in the MFC could use VBR as only electron donor for power generation. The matrix effects of the real wastewater on VBR removal and electricity generation of MFC were insignificant. VBR degradation by the electrogenic bacteria involves a stepwise demethylation process to yield partially dealkylated VBR species. In addition, these results demonstrate the feasibility of inoculating culturable bacteria strains to develop an efficient MFC for purifying wastewater.
本研究旨在从废水污泥中筛选产电细菌。系统发育分析表明，在有机污染物分解过程中，变形菌门是微生物燃料电池（MFC）中的优势菌门。五种可培养细菌菌株，即枯草芽孢杆菌、黄杆菌属、嗜水气单胞菌、弗氏柠檬酸杆菌和嗜麦芽窄食单胞菌，在染料去除和发电方面具有双重潜力。我们以特定比例接种混合产电细菌，并用三苯甲烷染料维多利亚蓝R（VBR）对其进行处理，以评估它们对人工废水和实际废水的发电能力。VBR冲击负荷实验结果表明，接种的MFC在1 - 2天内可适应冲击负荷，对100 - 800 mg/L的VBR表现出高去除效率（95 - 100%），功率密度为8.62±0.10至34.81±0.25 mW/m²。MFC中筛选出的产电细菌可将VBR仅作为电子供体用于发电。实际废水对MFC去除VBR和发电的基质效应不显著。产电细菌对VBR的降解涉及逐步脱甲基过程，以产生部分脱烷基的VBR物种。此外，这些结果证明了接种可培养细菌菌株以开发高效MFC用于净化废水的可行性。
