Department of Chemical Engineering & Technology, Centre of Advanced Study, Indian Institute of Technology, BHU, Varanasi, 221005, India.
Appl Biochem Biotechnol. 2020 Sep;192(1):196-210. doi: 10.1007/s12010-020-03306-w. Epub 2020 Apr 27.
Microbial fuel cell (MFC) is an attractive green technology which harnesses the power of microorganism for the production of electricity along with bioremediation of waste. However, the bioremediation of the high concentration of dye wastewater in MFC remains unclear. In present study, double-chambered MFC inoculated with mixed bacterial consortium was used for bioremediation of reactive orange 16 (RO-16) dyes at a very high and variable concentration range of 100 to 1000 ppm. Maximum voltage was obtained for 100 ppm of dye and was found to be 0.5791 V along with a power density of 0.0851 W/m. Till 500 ppm concentration of dye COD removal efficiency remains in range of 40 to 100% thereafter it decreases. The maximum concentration of CO was found to be 2% at 1000 ppm which confirms the biodegradation phenomena in MFC. Kinetics of biodegradation of reactive orange 16 were studied using Haldane inhibitory kinetic model and kinetic constants μ, K, and K were calculated and found to be 0.417 day, 206.2 ppm, and 447.12 ppm respectively. The experimental results showed inhibitory condition in the MFC after 500 ppm and it was supported by the value of inhibitory kinetic constant K = 447.12 ppm. This study opened the possibility of bioremediation of dyes at high concentrations in MFCs.
微生物燃料电池（MFC）是一种有吸引力的绿色技术，它利用微生物的力量来发电，同时对废物进行生物修复。然而，MFC 中高浓度染料废水的生物修复仍不清楚。在本研究中，采用双室 MFC 接种混合细菌菌剂，对浓度范围为 100 至 1000 ppm 的活性橙 16（RO-16）染料进行生物修复。在 100 ppm 的染料中获得最大电压，为 0.5791 V，同时功率密度为 0.0851 W/m。在 500 ppm 染料浓度下，COD 去除效率仍保持在 40%至 100%之间，之后下降。在 1000 ppm 时，发现最大 CO 浓度为 2%，这证实了 MFC 中的生物降解现象。使用 Haldane 抑制动力学模型研究了活性橙 16 的生物降解动力学，计算了动力学常数μ、K 和 K，并分别为 0.417 天、206.2 ppm 和 447.12 ppm。实验结果表明，在 500 ppm 后 MFC 中存在抑制条件，这得到了抑制动力学常数 K = 447.12 ppm 的支持。这项研究为 MFC 中高浓度染料的生物修复开辟了可能性。
