He Ping, Jin Xiaojie
Department of Otolaryngology, South Campus RenJi Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Biochem Cell Biol. 2018 Jun;96(3):326-331. doi: 10.1139/bcb-2017-0160. Epub 2017 Oct 16.
The aim of this study was to investigate the role of SOX10 in nasopharyngeal carcinoma (NPC) and the underlying molecular mechanisms.
The expression of SOX10 was initially assessed in human NPC tissues and a series of NPC cell lines through quantitative real-time PCR (qRT-PCR) and Western blot. Then, cell proliferation, cycle, migration, and the invasiveness of NPC cells with knockdown of SOX10 were examined by MTT, flow cytometry, and Transwell migration and invasion assays, respectively. Finally, nude mice tumorigenicity experiments were performed to evaluate the effects of SOX10 on NPC growth and metastasis in vivo.
SOX10 was significantly increased in NPC tissues and cell lines. In-vitro experiments revealed that loss of SOX10 obviously inhibited cell proliferation, migration, and invasiveness, as well as the epithelial-mesenchymal transition (EMT) process in NPC cells. In-vivo experiments further demonstrated that disrupted SOX10 expression restrained NPC growth and metastasis, especially in lung and liver.
Taken together, our data confirmed the role of SOX10 as an oncogene in NPC progression, and revealed that SOX10 may serve as a novel biomarker for diagnosis of NPC, as well as a potential therapeutic target against this disease.
本研究旨在探讨SOX10在鼻咽癌（NPC）中的作用及潜在分子机制。
首先通过定量实时PCR（qRT-PCR）和蛋白质免疫印迹法检测人鼻咽癌组织及一系列鼻咽癌细胞系中SOX10的表达。然后，分别采用MTT法、流式细胞术以及Transwell迁移和侵袭实验检测SOX10基因敲低的鼻咽癌细胞的增殖、周期、迁移和侵袭能力。最后，进行裸鼠致瘤性实验，以评估SOX10对鼻咽癌在体内生长和转移的影响。
SOX10在鼻咽癌组织和细胞系中显著上调。体外实验表明，SOX10缺失明显抑制鼻咽癌细胞的增殖、迁移和侵袭以及上皮-间质转化（EMT）过程。体内实验进一步证明，SOX10表达的破坏抑制了鼻咽癌的生长和转移，尤其是在肺和肝中的转移。
综上所述，我们的数据证实了SOX10作为一种癌基因在鼻咽癌进展中的作用，并表明SOX10可能作为鼻咽癌诊断的新型生物标志物以及针对该疾病的潜在治疗靶点。
