Department of Medical Biology, Faculty of Medicine, Sivas, Turkey.
Department of Cardiovascular Surgery, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.
Atherosclerosis. 2017 Nov;266:176-181. doi: 10.1016/j.atherosclerosis.2017.10.012. Epub 2017 Oct 7.
Genetic and environmental factors are important components of the development of atherosclerosis. Long non-coding RNA (lncRNAs) have emerged as regulators of multiple pathophysiological pathways in the cardiovascular system. Here, we investigated potential associations between lncRNAs and atherosclerosis.
Tissue samples from atherosclerotic coronary artery plaques and non-atherosclerotic internal mammary artery were obtained from 20 patients during coronary artery bypass surgery. Expression levels of five lncRNAs known to be associated with coronary artery disease were measured using quantitative PCR.
Cyclin-dependent kinase inhibitor 2B antisense RNA 1 (ANRIL) and myocardial infarction-associated transcript (MIAT) were more expressed in the atherosclerotic arteries compared to the non-atherosclerotic arteries. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) was less expressed in the atherosclerotic plaques. Expression levels of potassium voltage-gated channel, KQT-like subfamily, member 1 opposite strand/antisense transcript 1 (KCNQ1OT1) and hypoxia inducible factor 1A antisense RNA 2 (aHIF) were comparable between atherosclerotic and non-atherosclerotic arteries. In the atherosclerotic plaque, expression levels of MALAT1, MIAT, KCNQ1OT1 and aHIF were inversely correlated with age.
We report significant associations between lncRNAs and atherosclerosis. These findings support a role for lncRNAs in coronary artery disease development.
遗传和环境因素是动脉粥样硬化发展的重要组成部分。长链非编码 RNA（lncRNAs）已成为心血管系统中多种病理生理途径的调节因子。在这里，我们研究了 lncRNAs 与动脉粥样硬化之间的潜在关联。
在冠状动脉旁路手术期间，从 20 名患者的动脉粥样硬化性冠状动脉斑块和非动脉粥样硬化性内乳动脉组织中获取组织样本。使用定量 PCR 测量五种与冠状动脉疾病相关的 lncRNAs 的表达水平。
与非动脉粥样硬化性动脉相比，在动脉粥样硬化性动脉中，细胞周期蛋白依赖性激酶抑制剂 2B 反义 RNA 1（ANRIL）和心肌梗塞相关转录物（MIAT）的表达更高。在动脉粥样硬化斑块中，转移性肺腺癌转录物 1（MALAT1）的表达水平较低。钾电压门控通道，KQT 样亚家族，成员 1 反义链/反义转录物 1（KCNQ1OT1）和缺氧诱导因子 1A 反义 RNA 2（aHIF）的表达水平在动脉粥样硬化和非动脉粥样硬化性动脉之间无差异。在动脉粥样硬化斑块中，MALAT1、MIAT、KCNQ1OT1 和 aHIF 的表达水平与年龄呈负相关。
我们报告了 lncRNAs 与动脉粥样硬化之间的显著关联。这些发现支持 lncRNAs 在冠状动脉疾病发展中的作用。
