Çekin Nilgün, Özcan Arzu, Göksel Sabahattin, Arslan Serdal, Pınarbaşı Ergün, Berkan Öcal
Department of Medical Biology, Faculty of Medicine, Cumhuriyet University; Sivas-Turkey.
Anatol J Cardiol. 2018 Feb;19(2):131-136. doi: 10.14744/AnatolJCardiol.2017.8081.
Cardiovascular diseases are the most important cause of mortality worldwide, particularly atherosclerosis. Recently, lncRNAs affecting atherosclerotic progression have been reported in vascular smooth muscle cells, endothelial cells, and monocytes, suggesting that lncRNAs play an important role in atherosclerosis.
In recent clinical studies, nowadays, it was determined that internal mammary bypass grafts are closest to ideal grafts in coronary artery bypass surgery. In this study, we used tissue samples taken from atherosclerotic coronary arteries and the internal mammary artery (IMA) during coronary artery bypass surgery. Using RT-PCR, we investigated the role of two lncRNAs, FENDRR and LincRNA-p21, by comparing their expression levels in coronary artery plaques and normal mammary arteries of 20 atherosclerotic patients.
We found that the FENDRR and LincRNA-p21 expressions decreased by approximately 2 and 7 fold in coronary artery plaques, respectively, compared with those in IMA, which is known to have no plaque development.
This study was the first to use mammary artery tissues of the same patients as a control and to study FENDRR expression. Our data may provide helpful insights regarding the association of lncRNAs and atherosclerosis.
心血管疾病是全球最重要的死亡原因，尤其是动脉粥样硬化。最近，在血管平滑肌细胞、内皮细胞和单核细胞中报道了影响动脉粥样硬化进展的长链非编码RNA（lncRNAs），这表明lncRNAs在动脉粥样硬化中起重要作用。
在最近的临床研究中，现已确定在冠状动脉搭桥手术中，乳内动脉搭桥移植物最接近理想的移植物。在本研究中，我们使用了在冠状动脉搭桥手术期间从动脉粥样硬化冠状动脉和乳内动脉（IMA）获取的组织样本。通过逆转录聚合酶链反应（RT-PCR），我们比较了20例动脉粥样硬化患者冠状动脉斑块和正常乳内动脉中两种lncRNAs，即FENDRR和LincRNA-p21的表达水平，以研究它们的作用。
我们发现，与已知无斑块形成的IMA相比，冠状动脉斑块中FENDRR和LincRNA-p21的表达分别下降了约2倍和7倍。
本研究首次使用同一患者的乳内动脉组织作为对照，并研究FENDRR的表达。我们的数据可能为lncRNAs与动脉粥样硬化的关联提供有益的见解。
