Jeong Jae-Kwan, Choi In-Soo, Moon Sung-Ho, Lee Soo-Chan, Kang Hyun-Gu, Jung Young-Hun, Park Soo-Bong, Kim Ill-Hwa
College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, 28644, Republic of Korea.
National Institute of Animal Science, RDA, Cheonan, Chungnam, 31000, Republic of Korea.
Theriogenology. 2018 Jan 15;106:53-59. doi: 10.1016/j.theriogenology.2017.09.030. Epub 2017 Sep 25.
We determined the effect of ketosis treatment with propylene glycol (PG) or PG plus l-carnitine and methionine (Metabolase, Fatro, Bologna, Italy) on the resolution, postpartum health, milk yield, and reproductive performances of dairy cows. Blood from 475 Holstein cows was collected weekly until 4 weeks after calving to measure blood β-hydroxybutyrate (BHBA) concentrations. Cows with blood BHBA concentration ≥1.2 mmol/L were diagnosed with ketosis and were enrolled. One hundred and fifty cows diagnosed with ketosis were randomly assigned to three treatment groups (Day 0): (1) PG (300 g, PO) for 3 days (PG group, n = 50), (2) PG (300 g, PO) plus l-carnitine (1.25 g) plus methionine (5 g, IV) for 3 days (PG + CM group, n = 50), and (3) no treatment (control group, n = 50). On Day 3, blood was collected to evaluate whether the ketosis had resolved. Cows in the PG and PG + CM groups with blood BHBA ≥1.2 mmol/L were retreated for an additional 2 days, and then blood BHBA concentration was evaluated on Days 5 and 10. Blood glucose and haptoglobin concentrations, rumen fill score (RFS), and body condition score (BCS) were measured on Days 0, 3, 5, and 10. Postpartum complications, milk yield during the first 2 months, and reproductive outcomes were evaluated. The probability of resolution from ketosis was higher (P < 0.05) in the PG + CM group than in the control group on Days 3, 5, and 10 (odds ratio: 2.6-6.3). Blood BHBA in the PG + CM group was lower (P < 0.05) than that of the control group on Days 3 and 5, whereas blood glucose in the PG + CM group was higher (P < 0.05) than that of the control group on Days 3 and 5. RFS in the PG and PG + CM groups was higher than that of the control group on Day 10 (P < 0.01), while BCS loss from Day 0-10 in the control group was higher than those of the PG and PG + CM groups (P < 0.05). Milk yields on the 30th and 60th days postpartum were higher in the PG + CM group than the control and PG groups (P < 0.05). Postpartum complications and intervals between calving and first postpartum insemination or pregnancy did not differ among the groups (P > 0.05). In conclusion, treatment of dairy cows with PG plus l-carnitine and methionine improved the chances of resolution of ketosis and increased milk yield, while affecting neither the incidence of postpartum complications nor reproductive performance.
我们测定了用丙二醇（PG）或PG加左旋肉碱和蛋氨酸（Metabolase，法特罗公司，意大利博洛尼亚）进行酮症治疗对奶牛酮症消退、产后健康、产奶量和繁殖性能的影响。每周采集475头荷斯坦奶牛的血液，直至产犊后4周，以测定血液中β-羟基丁酸（BHBA）浓度。血液BHBA浓度≥1.2 mmol/L的奶牛被诊断为酮症并纳入研究。150头被诊断为酮症的奶牛被随机分为三个治疗组（第0天）：（1）PG（300 g，口服），持续3天（PG组，n = 50）；（2）PG（300 g，口服）加左旋肉碱（1.25 g）加蛋氨酸（5 g，静脉注射），持续3天（PG + CM组，n = 50）；（3）不治疗（对照组，n = 50）。在第3天采集血液，以评估酮症是否已消退。PG组和PG + CM组中血液BHBA≥1.2 mmol/L的奶牛再接受2天治疗，然后在第5天和第10天评估血液BHBA浓度。在第0、3、5和10天测量血糖和触珠蛋白浓度、瘤胃充盈评分（RFS）和体况评分（BCS）。评估产后并发症、前两个月的产奶量和繁殖结果。在第3、5和10天，PG + CM组酮症消退的概率高于对照组（P < 0.05）（优势比：2.6 - 6.3）。在第3天和第5天，PG + CM组的血液BHBA低于对照组（P < 0.05），而在第3天和第5天，PG + CM组的血糖高于对照组（P < 0.05）。在第10天，PG组和PG + CM组的RFS高于对照组（P < 0.01），而对照组从第0天到第10天的BCS损失高于PG组和PG + CM组（P < 0.05）。PG + CM组产后第30天和第60天的产奶量高于对照组和PG组（P < 0.05）。各组间产后并发症以及产犊至首次产后输精或怀孕的间隔时间无差异（P > 0.05）。总之，用PG加左旋肉碱和蛋氨酸治疗奶牛可提高酮症消退的几率并增加产奶量，同时不影响产后并发症的发生率和繁殖性能。
