Varghese Anju, Raina Opinder K, Chandra Dinesh, Mirdha Bijay R, Kelawala Naresh H, Solanki Jayesh B, Kumar Niranjan, Ravindran Reghu, Arun Anandanarayanan, Rialch Ajayta, Lalrinkima Hniang, Kelawala Rohan N, Samanta Subhamoy
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Acta Parasitol. 2017 Dec 20;62(4):775-778. doi: 10.1515/ap-2017-0093.
Three recombinant antigens viz. arginine kinase, cathepsin L-1 and TES-26 of Toxocara canis were expressed in Escherichia coli and evaluated for their potential in the detection of T. canis larval infection in human in immunoglobulin G-enzyme linked immunosorbent assay (IgG-ELISA). Results of the IgG-ELISA with the above recombinant antigens were confirmed with commercially available IgG detection kit for T. canis infection used as a standard test. All three recombinant antigens were 100% sensitive in the detection of positive cases (n = 6) of T. canis infection in human and were screened for their cross-reactivity in human patients with history of Toxoplasma gondii, Plasmodium vivax, Entamoeba histolytica, hydatid and hookworm infections. The recombinant TES-26 antigen showed higher specificity and cross-reacted with T. gondii infection sera only. However, arginine kinase and cathepsin L-1 recombinant antigens showed cross-reactions with sera of patients infected with T. gondii, P. vivax and E. histolytica but not with the patient sera infected with hydatid and hookworm. These results show that recombinant TES-26 is a potential diagnostic candidate antigen for human toxocarosis caused by migrating T. canis larvae.
三种重组抗原，即犬弓首蛔虫的精氨酸激酶、组织蛋白酶L-1和TES-26，在大肠杆菌中表达，并在免疫球蛋白G酶联免疫吸附测定（IgG-ELISA）中评估其在检测人体犬弓首蛔虫幼虫感染方面的潜力。使用市售的用于犬弓首蛔虫感染的IgG检测试剂盒作为标准检测，对上述重组抗原进行IgG-ELISA检测结果进行了确认。所有三种重组抗原在检测人体犬弓首蛔虫感染阳性病例（n = 6）时的敏感性均为100%，并对有弓形虫、间日疟原虫、溶组织内阿米巴、包虫和钩虫感染史的人类患者进行了交叉反应性筛查。重组TES-26抗原显示出更高的特异性，仅与弓形虫感染血清发生交叉反应。然而，精氨酸激酶和组织蛋白酶L-1重组抗原与弓形虫、间日疟原虫和溶组织内阿米巴感染患者的血清发生交叉反应，但与包虫和钩虫感染患者的血清不发生交叉反应。这些结果表明，重组TES-26是由迁移的犬弓首蛔虫幼虫引起的人体弓首蛔虫病的潜在诊断候选抗原。
