Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Wayanad, Kerala, India.
Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
Acta Parasitol. 2022 Mar;67(1):523-529. doi: 10.1007/s11686-021-00464-7. Epub 2021 Aug 28.
Toxocara canis is a common intestinal nematode parasite of dogs with recognized zoonotic potential in tropical countries. The purpose of this study was to determine the seroprevalence of anti-T. canis antibodies in two target dog populations: household and community-owned, distributed over three distinct geographical regions of India.
Two recombinant proteins of T. canis, cathepsin L-1 (CL-1) and Toxocara excretory-secretory-26 (TES-26), expressed in Escherichia coli, were used for studying the prevalence of anti-T. canis antibodies in dog populations in three distinct geographical regions of the country using an IgG-enzyme-linked immunosorbent assay. A total of 615 sera, 507 from household and 108 from community owned dogs were screened for IgG antibodies.
ELISA with recombinant (r) CL-1 showed 37.7% and 53.7% seroreactivity in household and community owned dogs, respectively. However, the rTES-26 antigen showed higher seroreactivity of 39.6% and 87.9% in the corresponding groups of household and community owned dogs, respectively. Chi-squared analysis of the data indicated that there was not any association in the prevalence of anti-T. canis antibodies between the samples analyzed from the three regions and the two cohorts of dog groups. However, the seroprevalence was higher in community owned dogs compared to household owned dogs.
The results of the serological evaluation suggest that both the groups of dogs show high seroreactivity rates and are likely to harbor T. canis infections of tissue dwelling dormant larvae.
犬弓首蛔虫是一种常见的犬肠道寄生线虫，具有公认的热带国家的动物源性。本研究的目的是确定两种目标犬群体的抗犬弓首蛔虫抗体的血清流行率：家庭和社区拥有的犬，分布在印度三个不同的地理区域。
使用两种犬弓首蛔虫重组蛋白，即组织蛋白酶 L-1（CL-1）和犬弓首蛔虫排泄分泌-26（TES-26），在大肠杆菌中表达，用于使用 IgG 酶联免疫吸附试验研究该国三个不同地理区域的犬群体中抗犬弓首蛔虫抗体的流行率。共筛查了 615 份血清，其中 507 份来自家庭犬，108 份来自社区犬，用于 IgG 抗体。
用重组（r）CL-1 的 ELISA 显示家庭犬和社区犬的血清反应性分别为 37.7%和 53.7%。然而，rTES-26 抗原在相应的家庭犬和社区犬组中显示出更高的血清反应性，分别为 39.6%和 87.9%。数据的卡方分析表明，来自三个区域的样本与两个犬群的分析之间，抗犬弓首蛔虫抗体的流行率没有任何关联。然而，社区犬的血清阳性率高于家庭犬。
血清学评估的结果表明，两组犬都显示出较高的血清反应性，并且可能都存在组织内休眠幼虫的犬弓首蛔虫感染。
