Santos Lucas Moreira Dos, de Moura Micaele Quintana, Azevedo Morgana Lüdtke, Marques Giuli Argou, Avila Luciana Farias da Costa, Scaini Carlos James, Berne Maria Elisabeth, Moreira Ângela Nunes, Conceição Fabricio Rochedo
Programa de Pós-Graduação em Biotecnologia, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brasil.
Programa de Pós-Graduação em Parasitologia, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brasil.
Parasite Immunol. 2018 Aug;40(8):e12568. doi: 10.1111/pim.12568.
While the use of recombinant antigens is being widely investigated in the diagnosis of human toxocariasis, relatively little attention has been given to animal diagnostic models. For this reason, this study aimed to investigate the diagnosis potential of Toxocara canis TES-30 and TES-120 recombinant antigens in mice, the animal model for toxocariasis studies.
Serum samples obtained from mice infected with T. canis or Toxocara cati were tested by indirect ELISA using T. canis TES-30 and TES-120 recombinant antigens produced in Escherichia coli. 90% of the samples reacted with rTES-30, whereas there was almost no reactivity with rTES-120.
Despite rTES-120 being a good antigen for diagnosis in humans, it could not reproduce its reactivity in this animal model. As rTES-30 has good reactivity in mice, it is a valuable tool for diagnosis.
虽然重组抗原在人体弓首蛔虫病诊断中的应用正在广泛研究，但动物诊断模型相对较少受到关注。因此，本研究旨在探讨犬弓首蛔虫TES-30和TES-120重组抗原在作为弓首蛔虫病研究动物模型的小鼠中的诊断潜力。
使用在大肠杆菌中产生的犬弓首蛔虫TES-30和TES-120重组抗原，通过间接ELISA对感染犬弓首蛔虫或猫弓首蛔虫的小鼠血清样本进行检测。90%的样本与rTES-30发生反应，而与rTES-120几乎没有反应。
尽管rTES-120在人体诊断中是一种良好的抗原，但在该动物模型中无法重现其反应性。由于rTES-30在小鼠中具有良好的反应性，它是一种有价值的诊断工具。
