Blaskovic D, Sekeyová Z, Turna J, Kúdelová M, Slávik I, Mucha V
Institute of Virology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Acta Virol. 1988 Jul;32(4):329-33.
Strain Sumava Af of murine alpha-herpesvirus (MHV) banded in density gradient at the boundary between 10-20% and 20-30% Ficoll solution. In electron microscope, the partially purified virus visualized by negative staining exhibited intact or disrupted envelope structures and capsid particles of about 110 nm in outer diameter. The DNA extracted from partially purified virus and further purified in CsCl density gradient had the Tm-value of 79.0 degrees C. The molecular weight of about 90 million daltons was calculated for viral DNA according to its migration in agarose gel.
鼠α-疱疹病毒（MHV）的苏马瓦毒株Af在密度梯度中于10%-20%和20%-30%的菲可溶液之间的边界处形成条带。在电子显微镜下，通过负染色观察到的部分纯化病毒呈现出完整或破裂的包膜结构以及外径约为110 nm的衣壳颗粒。从部分纯化病毒中提取并在CsCl密度梯度中进一步纯化的DNA的解链温度为79.0摄氏度。根据病毒DNA在琼脂糖凝胶中的迁移情况计算出其分子量约为9000万道尔顿。
