根癌土壤杆菌（史密斯和汤森）康恩噬菌体PS8的研究：其DNA的物理化学性质

Studies on the bacteriophage PS8 of Agrobacterium tumefaciens (Smith and Townsend) Conn: physico-chemical properties of its DNA.

作者信息

Knopf U C

出版信息

Microbios. 1976;17(70):231-7.

Abstract

DNA from the bacteriophage PS8 was extracted and purified. The buoyant density was determined was 1.716 cm3/g. The guanine-cytosine content was calculated to be 57%. DNA molecules which looked like circles were found among linear strands in an electron-microscopic study. With an endonuclease from Streptomyces albus G the DNA was digested to 19 fragments, with molecular weights ranging from 600 to 7,400 daltons. The molecular weight of the DNA was determined to be 38.8 X 10(6) daltons +/- 8.7%.

摘要

从噬菌体PS8中提取并纯化了DNA。测得其浮力密度为1.716立方厘米/克。计算得出鸟嘌呤-胞嘧啶含量为57%。在电子显微镜研究中，在线性链中发现了看起来像环状的DNA分子。用来自白色链霉菌G的一种内切核酸酶将该DNA消化成19个片段，分子量范围为600至7400道尔顿。测定该DNA的分子量为38.8×10⁶道尔顿±8.7%。