Kaviani Maryam, Ghaderian Sayyed Mohammad Hossein, Arefi Soheila, Hashemi Mehrdad, Afjeh Sarah Sadat Aghabozorg
Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Hum Antibodies. 2017;26(3):121-126. doi: 10.3233/HAB-170329.
Gonadotropin therapy was used to stimulate the ovary in infertile women who underwent assisted reproduction treatment (ART). Numerous studies indicated that infertile women showed different responses to gonadotropin therapy. Follicle stimulating hormone receptor (FSHR) and Oestrogen receptor (ER) polymorphisms have been reported to involve induction of folliculogenesis and ovarian response to treatment.
In the present study, two polymorphisms, namely FSHR rs6165 and ESR2 rs4986938, were investigated in 198 Iranian infertile women aged less than 39 years who underwent ART. After DNA extraction, these polymorphisms were genotyped by TaqMan genotyping assay. According to the number of eggs released during ovulation, the patients were categorized into poor responders (PR) and good responders (GR). The results indicated that the good responders showed lower levels of FSH and LH than poor responders.
No association was observed between ESR2 rs4986938 and poor response in Iranian women patients. Instead, FSHR rs6165 showed a strong association with ovarian response to ART (p< 0.05).
The obtained results indicated that FSHR rs6165, not ESR2 rs4986938, could be suggested as a candidate marker to predict poor ovarian response.
促性腺激素疗法用于刺激接受辅助生殖治疗（ART）的不孕女性的卵巢。大量研究表明，不孕女性对促性腺激素疗法表现出不同的反应。据报道，促卵泡激素受体（FSHR）和雌激素受体（ER）多态性参与卵泡发生的诱导和卵巢对治疗的反应。
在本研究中，对198名年龄小于39岁且接受ART的伊朗不孕女性进行了两项多态性研究，即FSHR rs6165和ESR2 rs4986938。提取DNA后，通过TaqMan基因分型测定法对这些多态性进行基因分型。根据排卵期间排出的卵子数量，将患者分为低反应者（PR）和高反应者（GR）。结果表明，高反应者的FSH和LH水平低于低反应者。
在伊朗女性患者中，未观察到ESR2 rs4986938与低反应之间的关联。相反，FSHR rs6165与ART的卵巢反应密切相关（p<0.05）。
所得结果表明，FSHR rs6165而非ESR2 rs4986938可作为预测卵巢低反应的候选标志物。
